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Team:Freiburg/main2
From 2013.igem.org
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<img id="cas" src="https://static.igem.org/mediawiki/2013/9/94/Freiburg_2013_main2_Cas.png"> | <img id="cas" src="https://static.igem.org/mediawiki/2013/9/94/Freiburg_2013_main2_Cas.png"> | ||
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<span> Activation </span> | <span> Activation </span> | ||
| - | <img | + | <img src="https://static.igem.org/mediawiki/2013/0/03/Freiburg_2013_main2_Activation.png"> |
</div> | </div> | ||
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<span> Epigenetics </span> | <span> Epigenetics </span> | ||
| - | <img | + | <img src="https://static.igem.org/mediawiki/2013/3/31/Freiburg_2013_main2_Epigenetic.png"> |
</div> | </div> | ||
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<span> Light switch </span> | <span> Light switch </span> | ||
| - | <img | + | <img src="https://static.igem.org/mediawiki/2013/9/9a/Freiburg_2013_main2_Licht.png"> |
</div> | </div> | ||
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<span> Multiple targeting </span> | <span> Multiple targeting </span> | ||
| - | <img | + | <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png"> |
</div> | </div> | ||
Revision as of 22:47, 26 September 2013
Abstract Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene regulation in mammalian cells. Therefore, we engineered the recently discovered and highly promising CRISPR/Cas9 system. The regulation is based on the RNA-guided Cas9 protein, which allows targeting of specific DNA sequences. Our toolkit comprises not only a standardized Cas9 protein, but also different effector domains for efficient gene activation or repression.
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Activation
Epigenetics
Light switch
Multiple targeting
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