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Team:Freiburg/parts/BioBricks
From 2013.igem.org
Our BioBricks
Welcome to our parts and devices page. Here you will find all components of our uniCAS toolkit for gene regulation. If you want to use our BioBricks, feel free to click through our online tool that generates a personal manual for your needs.
| Parts | Biobrick-Number | Length [bp] | Designer |
|---|---|---|---|
| Cas9 | BBa_K1150000 | 4101 | Team Freiburg 2013 |
| VP16 | BBa_K1150001 | 372 | Team Freiburg 2013 |
| KRAB | BBa_K1150002 | 366 | Team Freiburg 2013 |
| G9a | BBa_K1150003 | 840 | Team Freiburg 2013 |
| PhyB | BBa_K1150004 | 1950 | Team Freiburg 2013 |
| PIF6 | BBa_K1150005 | 300 | Team Freiburg 2013 |
| UVR8 | BBa_K1150006 | 1110 | Team Freiburg 2013 |
| COP1 | BBa_K1150007 | 1023 | Team Freiburg 2013 |
| NLS | BBa_K1150010 | 21 | Team Freiburg 2013 |
| SV40 Promotor RFC 10 | BBa_K1150011 | 344 | Team Freiburg 2013 |
| BGH Terminator RFC10 | BBa_K1150012 | 222 | Team Freiburg 2013 |
| 3 AS Linker | BBa_K1150013 | 9 | Team Freiburg 2013 |
| 10 AS Linker | BBa_K1150014 | 30 | Team Freiburg 2013 |
| HA tag | BBa_K1150016 | 27 | Team Freiburg 2013 |
| | ||||||
|---|---|---|---|---|---|---|
| Favorite | Description | Biobrick-Number | Length [bp] | Designer | Genebank files | Plasmid maps |
| Cas9 with CMV promoter | BBa_K1150017 | 5012 | Team Freiburg 2013 | Genebank | Map | |
| Cas9 with SV40 promoter | BBa_K1150018 | 4776 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS Activator (SV40 promoter) | BBa_K1150019 | 5173 | Team Freiburg 2013 | Genebank | Map | |
| Yes | uniCAS Activator (CMV promoter) | BBa_K1150020 | 5409 | Team Freiburg 2013 | Genebank | Map |
| uniCAS Repressor (SV40 promoter) | BBa_K1150021 | 5165 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS Repressor (CMV promoter) | BBa_K1150022 | 5401 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS Histone Modifier (SV40 promoter) | BBa_K1150023 | 5641 | Team Freiburg 2013 | Genebank | Map | |
| Yes | uniCAS Histone Modifier (CMV promoter) | BBa_K1150024 | 5877 | Team Freiburg 2013 | Genebank | Map |
| uniCAS Red Light Switch Part I - Stimulator | BBa_K1150025 | 5335 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS Red Light Switch Part II - Activator | BBa_K1150026 | 3192 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS Red Light Switch Part II - Repressor | BBa_K1150027 | 3184 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS Red Light Switch Part II - Histone Modificator | BBa_K1150028 | 3660 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS UV Light Switch Part I - Stimulator | BBa_K1150029 | 6147 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS UV Light Switch Part II - Activator | BBa_K1150030 | 2265 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS UV Light Switch Part II - Repressor | BBa_K1150031 | 2257 | Team Freiburg 2013 | Genebank | Map | |
| uniCAS UV Light Switch Part II - Histone Modificator | BBa_K1150032 | 2733 | Team Freiburg 2013 | Genebank | Map | |
| Constitutive GFP Reporter | BBa_K1150033 | 1711 | Team Freiburg 2013 | |||
| Yes | dCAS RNA plasmid: "uniCAS RNaimer" | BBa_K1150034 | 815 | Team Freiburg 2013 | ||
| RNAimer containing EMX1 locus | BBa_K1150035 | 815 | Team Freiburg 2013 | |||
| RNAimer containing VEGF1 locus | BBa_K1150036 | 815 | Team Freiburg 2013 | |||
| RNAimer containing VEGF2 locus | BBa_K1150037 | 815 | Team Freiburg 2013 | |||
| RNAimer containing VEGF3 locus | BBa_K1150038 | 815 | Team Freiburg 2013 | |||
| RNAimer containing VEGF4 locus | BBa_K1150039 | 815 | Team Freiburg 2013 | |||
| RNAimer containing bla target 2 locus | BBa_K1150040 | 815 | Team Freiburg 2013 | |||
| RNAimer containing bla target 3 locus | BBa_K1150041 | 815 | Team Freiburg 2013 | |||
| RNAimer containing bla target 4 locus | BBa_K1150042 | 815 | Team Freiburg 2013 | |||
| RNAimer containing VEGF2 and VEGF 3 loci | BBa_K1150043 | 815 | Team Freiburg 2013 | |||
| RNAimer containing bla target 3 and 4 | BBa_K1150044 | 815 | Team Freiburg 2013 | |||
| RNAimer containing VEGF4 and bla target 2 loci | BBa_K1150045 | 815 | Team Freiburg 2013 | |||
| RNAimer containing bla target 2,3,4 and VEGF4 loci | BBa_K1150046 | 815 | Team Freiburg 2013 | |||
| Truncated CMV Cas9 Device #1 | BBa_K1150047 | 4676 | Team Freiburg 2013 | |||
| Truncated CMV Cas9 Device #2 | BBa_K1150048 | 4706 | Team Freiburg 2013 | |||
| Truncated CMV Cas9 Device #3 | BBa_K1150049 | 3881 | Team Freiburg 2013 | |||
| Truncated CMV Cas9 Device #4 | BBa_K1150050 | 3626 | Team Freiburg 2013 | |||
| Truncated CMV Cas9 Device #5 | BBa_K1150051 | 3722 | Team Freiburg 2013 | |||
| NLS-BGH terminator | BBa_K1150062 | 251 | Team Freiburg 2013 | |||
| HA-NLS-Cas9-Linker 3 AA | BBa_K1150063 | 4182 | Team Freiburg 2013 | |||
