Team:Evry/Notebook/w16
From 2013.igem.org
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<h2 align="center"> 4<sup>th</sup> October </h2> | <h2 align="center"> 4<sup>th</sup> October </h2> | ||
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+ | <h2>Microfluidic experiment</h2> | ||
+ | <p><b>30/09/13</b><br/> | ||
+ | Chip's channel were full of bacteria, even after 2 hours of washing <i>(with free iron M9)</i> because we let the bacteria growth over the week-end <i>(in LB medium)</i>. Hence, we prepared another chip to launch another experiment on the next morning.<br/> | ||
+ | As our bacteria have a plasmid AceB-sfGFP and there is no iron in our M9, we still check if bacteria expressed sfGFP protein.<br/> | ||
+ | The first results appear promising, but we need to confirm them with a proper experiment. | ||
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+ | |||
+ | <div align="center"> | ||
+ | <div class="captionedPicture" style="width:40%;float:center;"> | ||
+ | <a title="Bacteria expressing sfGFP, 1st experiment" href="https://static.igem.org/mediawiki/2013/thumb/f/fd/1st_Microfluidic_experiment.jpg/600px-1st_Microfluidic_experiment.jpg"> | ||
+ | <img alt="Bacteria expressing sfGFP, 1st experiment" src="https://static.igem.org/mediawiki/2013/thumb/f/fd/1st_Microfluidic_experiment.jpg/600px-1st_Microfluidic_experiment.jpg" class="Picture"/> | ||
+ | </a> | ||
+ | <div class="caption"> | ||
+ | <b>Figure 1:</b> <i>Escherichia coli</i> (TOP 10 strain) expressing sfGFP.<br/> | ||
+ | At the top of the picture, we can see that the channel is full of bacteria. | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
<div align="center"><img src="https://static.igem.org/mediawiki/2013/9/95/WikiFreeze.jpg"/></div> | <div align="center"><img src="https://static.igem.org/mediawiki/2013/9/95/WikiFreeze.jpg"/></div> |
Revision as of 21:38, 30 September 2013
Week 16: 30th September - 6th October
4th October
Microfluidic experiment
30/09/13
Chip's channel were full of bacteria, even after 2 hours of washing (with free iron M9) because we let the bacteria growth over the week-end (in LB medium). Hence, we prepared another chip to launch another experiment on the next morning.
As our bacteria have a plasmid AceB-sfGFP and there is no iron in our M9, we still check if bacteria expressed sfGFP protein.
The first results appear promising, but we need to confirm them with a proper experiment.