Team:Paris Saclay/Notebook/June/24

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(Difference between revisions)
(→Lab work)
 
Line 5: Line 5:
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- Order of oligonucleotides and strains  
+
- Order oligonucleotides and strains  
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- Research the intergenic sequence which contain the promoter for pseudomonas pseudoalcaligenes KF 707  
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- Research the intergenic sequence which contain the promoter for ''Pseudomonas pseudoalcaligenes'' KF 707  
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- Research on rhodococcus rhodoccrous RHA1
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- Research on ''Rhodococcus rhodoccrous'' RHA1
- Research on Registry of standard biological parts
- Research on Registry of standard biological parts
Line 17: Line 17:
- Code: BBa_ I722017. We don’t get it in the kit therefore we could ask to iGEM.
- Code: BBa_ I722017. We don’t get it in the kit therefore we could ask to iGEM.
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- Designing the Pndh* promoter primers called pfrn_ up and pfnr_down for respectively the forward and the reverse
+
- Design the Pndh* promoter primers called pfrn_ up and pfnr_down for forward and reverse primer, respectively
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- Listing the BioBricks which we may have a need
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- List the BioBricks which we may have a need
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- Planning the first part manipulation (the first and second BBa)  
+
- Plan the first part manipulation (the first and second BBa)  
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- Control and verification if we have some BioBrick with RBS and Terminators that could be used after
+
- Control and verify the construction if we have some BioBrick with RBS and Terminators that could be used after
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- General presentation about PCBs for Wiki written in Word
+
- General presentation written in Word about PCBs for Wiki
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- 3 ml of culture of E.Coli DH5α in LB prepared for overnight culture at 17h
+
- Prepare 3 ml overnight culture of E.Coli DH5α in LB medium

Latest revision as of 00:22, 5 October 2013

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Notebook : June 24

Lab work

- Order oligonucleotides and strains

- Research the intergenic sequence which contain the promoter for Pseudomonas pseudoalcaligenes KF 707

- Research on Rhodococcus rhodoccrous RHA1

- Research on Registry of standard biological parts

- Search and document about the BioBrick which contain the lacZ with RBS and terminator

- Code: BBa_ I722017. We don’t get it in the kit therefore we could ask to iGEM.

- Design the Pndh* promoter primers called pfrn_ up and pfnr_down for forward and reverse primer, respectively

- List the BioBricks which we may have a need

- Plan the first part manipulation (the first and second BBa)

- Control and verify the construction if we have some BioBrick with RBS and Terminators that could be used after

- General presentation written in Word about PCBs for Wiki

- Prepare 3 ml overnight culture of E.Coli DH5α in LB medium


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