"
Team:INSA Toulouse/contenu/project/biological construction/logic gates
From 2013.igem.org
Mesnageclem (Talk | contribs) |
Mesnageclem (Talk | contribs) |
||
| Line 71: | Line 71: | ||
| - | . | + | .and1anim a { |
display:block; | display:block; | ||
width:340px; | width:340px; | ||
| Line 78: | Line 78: | ||
background-repeat:no-repeat; | background-repeat:no-repeat; | ||
} | } | ||
| - | . | + | .and1anim a:hover { |
display:block; | display:block; | ||
width:340px; | width:340px; | ||
height: 139px; | height: 139px; | ||
background-image: url(https://static.igem.org/mediawiki/2013/c/c7/AND1_-340px.gif); | background-image: url(https://static.igem.org/mediawiki/2013/c/c7/AND1_-340px.gif); | ||
| + | background-repeat:no-repeat; | ||
| + | } | ||
| + | |||
| + | |||
| + | .and2anim a { | ||
| + | display:block; | ||
| + | width:340px; | ||
| + | height: 195px; | ||
| + | background-image: url(https://static.igem.org/mediawiki/2013/9/9d/AND2-00_-_340px.png); | ||
| + | background-repeat:no-repeat; | ||
| + | } | ||
| + | .and2anim a:hover { | ||
| + | display:block; | ||
| + | width:340px; | ||
| + | height: 195px; | ||
| + | background-image: url(https://static.igem.org/mediawiki/2013/a/a4/AND2_-340px.gif); | ||
background-repeat:no-repeat; | background-repeat:no-repeat; | ||
} | } | ||
| Line 118: | Line 134: | ||
<br>It needs both recombinases production to transcript the downstream gene.</p> | <br>It needs both recombinases production to transcript the downstream gene.</p> | ||
| - | <p class=" | + | <p class="and1anim"> |
<a href="#" class="imgcontentleft"></a> | <a href="#" class="imgcontentleft"></a> | ||
</p> | </p> | ||
| Line 128: | Line 144: | ||
<br>At first, both the promoter and the gene (with its own rbs and terminator) are on the wrong way and are not fonctionnal. Both are surrounded by recombination sites recognized by two different recombinases (Phic31 and FimE) | <br>At first, both the promoter and the gene (with its own rbs and terminator) are on the wrong way and are not fonctionnal. Both are surrounded by recombination sites recognized by two different recombinases (Phic31 and FimE) | ||
<br><br><img src="https://static.igem.org/mediawiki/2013/8/88/AND2_solo_-_340px.png" class="imgcontent" /> | <br><br><img src="https://static.igem.org/mediawiki/2013/8/88/AND2_solo_-_340px.png" class="imgcontent" /> | ||
| - | <br>It needs both recombinases (the purple one and the green one) to transcript the gene. | + | <br>It needs both recombinases (the purple one and the green one) to transcript the gene.<p> |
| - | + | ||
| + | <p class="and2anim"> | ||
| + | <a href="#" class="imgcontentleft"></a> | ||
| + | </p> | ||
| + | |||
| + | <p class="texteright"> <i> Move the mouse over the figure to see how it work! </i></p> | ||
| + | <div class="clear"></div> | ||
<br> | <br> | ||
Revision as of 09:28, 3 September 2013
Biological Construction
Logic Gates
An electronic full adder is composed of 5 logic gates (2 XOR, 2 AND and 1 OR)
To transpose these logic gates into biological gates, two publications published this year (2013) inspired us. (Siuti and al. 2013, Bonnet and al. 2013 )
These two publications were using recombinases to create biological gates.
The E.calculus full adder uses different recombinases : TP901.1, Bxb1, PhiC31 and FimE.
Each recombinase is a one way recombinase, it means that its recombination sites have been designed to ensure an one-way switch only. When a sequence between two recombination sites have been switched, the sites are not retained and the recombinase can not switch a second time.
The two AND gates
In a biological way, with the use of recombinases, an AND gate can be represented by different ways.
The E.calculus full adder uses two different AND gates.
The first one is composed of one promoter followed by two transcriptional terminators, each one surounded by recombination sites. Each terminator is surrounded by a different recombination site, recognized by two different recombinases : Bxb1 and TP 901.1.
Those two terminators are then followed by the downstream gene (with its own rbs and terminator).
If there is one input, the corresponding recombinase will be produced (the red one or the blue one), so one or the other terminator will be switched off and can then let the polymerase go.
It needs both recombinases production to transcript the downstream gene.
Move the mouse over the figure to see how it work!
For the other AND gate, it works the same way but does not use terminators. The entire gene has been put between recombiation sites.
At first, both the promoter and the gene (with its own rbs and terminator) are on the wrong way and are not fonctionnal. Both are surrounded by recombination sites recognized by two different recombinases (Phic31 and FimE)
It needs both recombinases (the purple one and the green one) to transcript the gene.
Move the mouse over the figure to see how it work!
The two XOR gates
As for the AND gates, the E.calculus full adder uses two different XOR gates.
The first one works the same way as the first AND gate. It is composed of one terminator surrounded by two different recombination sites (Bxb1 and TP901.1). The used sites are the same as for the first AND gate because the input are the same.
If there is one input, the corresponding recombinase will be produced (the red one or the blue one), so the terminator will be switched off and the downstream gene can be transcripted.
When there is two inputs, the terminator is switched twice and does not let the polymerase go
image effet hover
The second XOR gate works the same way as the second AND gate.
The gene is put the wrong way and surrounded by two differents recombination sites (PhiC31 and FimE)
The gene needs to be switched only once to be transcripted.
image effet hover
The full adder combined those 4 logic gates. (See Full adder)
