Team:UFMG Brazil/humanpractice
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The fluorimeter is a device capable of measuring the intensity of fluorescence emitted by a particular substance, so[[File:prototipo1.jpg|right|300px|thumb|right|First prototype]] we can be be able to know the exact amount of this substance in the sample. The bacteria that is being developed as a diagnostic tool for team-UFMG (CardBio project), will be able to report, according to the fluorescence intensity, the amount of certain substances (biomarkers) in serum of the patient, allowing a more accurate assessment of their health conditions. Despite its simplicity, the fluorometers are expensive equipaments and not so easy to use, so, there is a necessity to develop simple and cheaper model. | The fluorimeter is a device capable of measuring the intensity of fluorescence emitted by a particular substance, so[[File:prototipo1.jpg|right|300px|thumb|right|First prototype]] we can be be able to know the exact amount of this substance in the sample. The bacteria that is being developed as a diagnostic tool for team-UFMG (CardBio project), will be able to report, according to the fluorescence intensity, the amount of certain substances (biomarkers) in serum of the patient, allowing a more accurate assessment of their health conditions. Despite its simplicity, the fluorometers are expensive equipaments and not so easy to use, so, there is a necessity to develop simple and cheaper model. | ||
- | Based on previous iGEM projects[1] we design and build a portable and low cost fluorometer, that could be used | + | Based on previous iGEM projects[1] we design and build a portable and low cost fluorometer, that could be used with the Cardbio bacteria. The first version is based in the project of the Cambridge Team 2010 (Eglomer), but we use a cheaper light sensor: the TSL235, it´s a light-to-frequency converter that outputs a square wave (50% duty cycle) with frequency directly proportional to light intensity (irradiance). As you can see in the video below, which uses an open hardware oscilloscope to test the sensor: |
<center><html><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/sGH6udNYjFM?version=3&hl=en_US"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/sGH6udNYjFM?version=3&hl=en_US" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></html></center> | <center><html><object width="640" height="480"><param name="movie" value="//www.youtube.com/v/sGH6udNYjFM?version=3&hl=en_US"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="//www.youtube.com/v/sGH6udNYjFM?version=3&hl=en_US" type="application/x-shockwave-flash" width="640" height="480" allowscriptaccess="always" allowfullscreen="true"></embed></object></html></center> | ||
In the second version, we inserted a color sensor in the previous project (ADJD-S311, able to discriminate different light frequencies), we remove the keypad and use a larger screen for easier reading. | In the second version, we inserted a color sensor in the previous project (ADJD-S311, able to discriminate different light frequencies), we remove the keypad and use a larger screen for easier reading. | ||
- | <center>[[File:prototipo3.jpg|400px]]</center> | + | <center>[[File:prototipo3.jpg|400px|thumb|right|The box design]]</center> |
- | <center>[[File:EsquemaUFMG.jpg|400px]]</center> | + | <center>[[File:EsquemaUFMG.jpg|400px|thumb|center|Wiring diagram]]</center> |
- | <center>[[File:prototipo2.jpg|400px]]</center> | + | <center>[[File:prototipo2.jpg|400px|thumb|center|Second prototype]]</center> |
References [1]: | References [1]: |
Revision as of 00:19, 28 September 2013