Team:Freiburg/Highlights/2
From 2013.igem.org
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+ | Activation | ||
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+ | For activation we choosed VP-16 as effector. Through the transactivating function of VP-16 the expression of these genes will be enhanced.<br> | ||
+ | We achieved up to 30-fold activation. | ||
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+ | Chromatin modification (Repression) | ||
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+ | Specific chromatin modification was achieved by fusing the histone methyltransferase G9a to dCas9. With this protein we are able to specifically repress endogenous gene expression. | ||
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Revision as of 12:02, 4 October 2013
HIGHLIGHTS