Electro-Elution

1. Cut the strip under the UV plate, cut the gel in little pieces

2. Fill the vat with TAE 0.5X

3. Close one of the well of the saline bridge with a semi-permeable membrane, put the DNA in the other well

4. Apply a 400V current after closing the vat, during 30min

5. Reverse the polarity during 20s, get back the solution and throw it in a new tube

6. Add 200µL of water and 600µL of phenol chloroform

7. Vortex during 5min

8. Get back the aqueous phase

9. Precipitation with ethanol