"
Team:Freiburg/Highlights
From 2013.igem.org
HIGHLIGHTS
Discover a complete new breakthrough in the way you regulate your genes and feel the fascination of simple complexity.
NEW - NOW - RIGHT HERE
Get the uniCAS toolkit and be part of a fast growing new community of next generation scientific researchers. Discover the great possibilities of uniCAS Toolkit right below!
|
6 Reasons for loving the uniCAS toolkit All you need to realize your personal experiments are 6 smart amazingly easy to use tools that come with the uniCAS toolkit. Just use our custom-tailored uniTOOL to generate individual manuals. Best of all: Its all open source and non commercial! |
|
|
|
Cas9 makes it possible!
First derived from bacterial immune system this wonderful enzyme is a real multifunctional talent. Specific targeting of DNA has never been easier!
|
|
Activation The activation domain VP16 is able to activate transcription of genes. |
|
|
Repression The fusion of the transcriptional repressor domain KRAB leads to synthetic repression of gene expression. |
|
Chromatin modification (Repression) Specific chromatin modification was achieved by fusing a histone methyltransferase G9a to dCas9. With this protein we are able to specifically repress endogenous gene expression. |
|
|
uniBAss - Binding Assay We developed an ELISA based method. With this method we can quantify the binding efficiency of our proteins. We called this binding assay uniBAss. It is a powerful tool for the characterization of the interaction between the modified dCas9 and the locus specific RNA. |
