Team:Evry
From 2013.igem.org
Welcome to the Iron coli Project Home Page
Authors
E. van der Kouwe1, 2, G. Mercy3, B. Baudu4, A. Chhun1, F. Amiot1, G. Guillocheau1, L. Le Goff5, H. Léger5, L. Ujéda6, 11, W. Rostain7, C. Pauthenier8, T. Cerisy9, P. Parutto8, A. Jaramillo7, A. Tolonen9 and N. Pollet10
Abstract
This year, our project focuses on hematological disorders. More specifically, we focus on diseases that are subsequent to an iron overload such as hemochromatosis and thalassemia. For hereditary hemochromatosis, the symptoms are due to the overabsorption of iron from the duodenum by the mutation of the HFE protein. However, in case of thalassemia, patients excessively absorb iron because of a subsequent metabolic perturbation.
Nowadays, the iron overload is mainly treated by bloodlettings for hemochromatosic patients but cannot be extended to thalassemic patients who suffer from anaemia. The aim of our project is to prevent the intestinal absorption of iron, thus acting directly at the source.
Using the Ferric Uptake Regulation (FUR) system that controls siderophore biosynthesis (iron chelator), we engineer Escherichia coli in order to produce these siderophores in response of high concentrations of iron. To reduce the patient's iron absorption, our bacteria will be placed in a capsule and will be ingested during a meal. Once it will arrive in the duodenum, our bacteria will produce the siderophore at their full potential and chelate the iron.
Affiliations
1Université Evry Val d'Essonne
2Faculté de Pharmacie de Lyon
3Sup'Biotech Paris
4AgroParisTech
5Ecole Nationale Supérieure d'Informatique pour l'Industrie et l'Entreprise
6Sciences Po Paris
7Synth Bio Team, Institute of Systems and Synthetic Biology
8Bio-RetroSynth Team, Institute of Systems and Synthetic Biology
9LGBM, Genoscope
10Metamorphosys, Institute of Systems and Synthetic Biology
11Paris-Descartes