Team:British Columbia/Notebook/CRISPR

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Team CRISPR

July 3

List of BioBricks that are/will be on the way:

Cas9:

  • Cas9 - Joe and Anna
  • pTet -
  • const. promoter + Cas9 - Joe
  • promoter + Cas9 + Leader + R + spacer + R (on Kan)
  • L + R + spacer + R

Target:

  • tracerRNA
  • repeat + spacer + repeat - Vanins*2 + underlings
  • leader - Ray, Fisal, Dan

Decoy:

  • PAM + spacer (on Amp)


July 10th

Experimenter:Anna Müller

"Aim:" PCR the cas9 gene from Streptococcus thermophilus cells.

Results: Unsuccessful no bands were seen on the agarose gel.

September 22nd

Experimenter: Anna Müller

Aim: Digest T4, T7 spacer sequence with PAM site(decoy construct) with EcoRI and SpeI to later on ligate into plasmid with ampicillin resistance.

Results: will follow.


Aim: Digest PsBIA3 plasmid with ampicillin resistance with EcoRI and SpeI to construct the decoy plasmides.

Results: will follow.