Revision as of 20:26, 2 October 2013

Home
Project
Data page
Experimental results
Model
Lab work
Safety
Human practice
Team
Achievements
[http://2013.igem.org/wiki/index.php?title=Special:UserLogin&returnto=Team:ETH_Zurich/Data_Page Login]
WIKI
- Log out
- My account
- [http://2013.igem.org/wiki/index.php?title=Team:ETH_Zurich/Data_Page&action=edit Edit page]
- [http://2013.igem.org/wiki/index.php?title=Team:ETH_Zurich/Data_Page&action=history History]
- [http://2013.igem.org/Special:Upload Upload new file]

Gene circuit

Our favorite new parts

1. [http://parts.igem.org/Part:BBa_K1216002 Main Page] - Acetyl esterase (AES), BBa_K1216002 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate
2. [http://parts.igem.org/Part:BBa_K1216005 MainPage] - Alkaline phosphatase with His tag and TEV cleavage site (phoA), BBa_K1216002 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate
3. [http://parts.igem.org/Part:BBa_K1216007 MainPage] - pLuxR1 mutated promoter, BBa_K1216007 : the promoter has an EC₅₀ of 6.462 nM in liquid culture. This sensitivity is shifted 300'000 fold compared to the original BBa_R0062 pLuxR. The data were obtained by single cell analysis using a FACS device.

Characterized pre-existing parts :

1. [http://parts.igem.org/Part:BBa_R0062:Experience Experience] - pLuxR wild type, BBa_R0062, Antiquity (2003-01-31) : the promoter has an EC₅₀ of 0.02 nM in liquid culture and 4.45 nM on agar plates. The data were obtained by single cell analysis using a FACS device.
2. [http://parts.igem.org/Part:BBa_J61032:Experience Experience] - Alkaline phosphatase, BBa_J61032, Arkin Lab(2006-11-10) : phoA gene originated from Citrobacter. We did Michealis-Menten kinetics of the phoA enzyme and also showed the conversion of PNP (para-nitrophenol phosphate) in a yellow analog precipitate

Characterized new parts

1. [http://parts.igem.org/Part:BBa_K1216006 Main Page] - Acetyl esterase (AES)with His-Tag and TEV cleavage site, BBa_K1216006 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 5-Bromo-6-Chloro-3-indoxyl butyrate into an indigo analog precipitate
2. [http://parts.igem.org/Part:BBa_K1216000 MainPage] - β-Glucuronidase (gusA), BBa_K1216000 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.
3. [http://parts.igem.org/Part:BBa_K1216004 MainPage] - β-Glucuronidase (gusA)with HIS-Tag and TEV cleavage site, BBa_K1216004 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.
4. [http://parts.igem.org/Part:BBa_K1216003 MainPage] - β-N-Acetylglucosaminidase (nagZ), BBa_K1216003 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert ______ into an red analog precipitate.

We thank our sponsors:

@@ Line 8: / Line 8: @@
 <img src="https://static.igem.org/mediawiki/2013/1/1e/Datapage_v1.png" width="97%" usemap="map4" id="map4"/>
 <map id="map4" name="map4"><area shape="poly" alt="" title="" coords="76,77,129,63,120,45,160,73,138,113,129,93,81,110" href="http://parts.igem.org/Part:BBa_J09855" target="" /><area shape="rect" alt="" title="" coords="170,56,239,92" href="http://parts.igem.org/Part:BBa_J09855" target="" /><area shape="rect" alt="" title="" coords="150,205,236,243" href="http://parts.igem.org/Part:BBa_K1216002" target="" /><area shape="rect" alt="" title="" coords="898,56,973,93" href="http://parts.igem.org/Part:BBa_J09855" target="" /><area shape="rect" alt="" title="" coords="903,206,988,243" href="http://parts.igem.org/Part:BBa_K1216002" target="" /><area shape="rect" alt="" title="" coords="914,436,994,480" href="http://parts.igem.org/Part:BBa_J09855" target="" /><area shape="rect" alt="" title="" coords="925,588,1011,623" href="http://parts.igem.org/Part:BBa_K1216002" target="" /><area shape="rect" alt="" title="" coords="339,604,442,644" href="http://parts.igem.org/Part:BBa_K805016" target="" /><area shape="rect" alt="" title="" coords="337,461,431,499" href="http://parts.igem.org/Part:BBa_K1216003" target="" /><area shape="poly" alt="" title="" coords="251,58,299,53,303,38,336,76,303,107,303,92,250,90,248,84" href="http://parts.igem.org/Part:BBa_J23100" target="" /><area shape="poly" alt="" title="" coords="348,56,334,93,420,117,430,81" href="http://parts.igem.org/Part:BBa_K1216001" target="" /><area shape="poly" alt="" title="" coords="65,235,112,217,107,198,150,223,131,265,126,250,76,265" href="http://parts.igem.org/Part:BBa_K1216007" target="" /><area shape="poly" alt="" title="" coords="243,209,289,206,293,189,329,227,295,260,294,242,240,241" href="http://parts.igem.org/Part:BBa_R006" target="" /><area shape="poly" alt="" title="" coords="341,207,326,241,419,270,430,235,430,235" href="http://parts.igem.org/Part:BBa_K1216000" target="" /><area shape="poly" alt="" title="" coords="806,76,854,65,856,49,895,78,869,116,862,98,812,106" href="http://parts.igem.org/Part:BBa_J09855" target="" /><area shape="poly" alt="" title="" coords="984,59,1034,58,1034,43,1070,78,1037,110,1035,91,980,91" href="http://parts.igem.org/Part:BBa_J23100" target="" /><area shape="poly" alt="" title="" coords="812,225,859,214,858,198,904,229,871,268,868,250,816,257" href="http://parts.igem.org/Part:BBa_K1216007" target="" /><area shape="poly" alt="" title="" coords="996,208,1051,206,1050,191,1085,226,1051,258,1049,241,994,242" href="http://parts.igem.org/Part:BBa_R0062" target="" /><area shape="poly" alt="" title="" coords="1005,447,1051,441,1056,427,1091,464,1058,496,1057,478,998,478" href="http://parts.igem.org/Part:BBa_J23100" target="" /><area shape="poly" alt="" title="" coords="826,460,874,450,871,434,913,462,885,498,881,485,828,493" href="http://parts.igem.org/Part:BBa_J09855" target="" /><area shape="poly" alt="" title="" coords="1099,443,1086,477,1177,503,1184,467,1184,467" href="http://parts.igem.org/Part:BBa_K1216001" target="" /><area shape="poly" alt="" title="" coords="1077,56,1063,85,1150,119,1160,83" href="http://parts.igem.org/Part:BBa_K1216001" target="" /><area shape="poly" alt="" title="" coords="1090,205,1077,240,1164,269,1176,236" href="http://parts.igem.org/Part:BBa_K1216000" target="" /><area shape="poly" alt="" title="" coords="1021,597,1070,593,1070,578,1108,614,1074,647,1072,632,1016,628" href="http://parts.igem.org/Part:BBa_R0062" target="" /><area shape="poly" alt="" title="" coords="835,602,881,593,884,578,924,608,895,645,889,627,834,635" href="http://parts.igem.org/Part:BBa_K1216007" target="" /><area shape="poly" alt="" title="" coords="245,464,295,459,298,444,333,481,299,512,299,497,244,496" href="http://parts.igem.org/Part:BBa_J23100" target="" /><area shape="poly" alt="" title="" coords="245,606,297,603,296,587,333,624,301,657,296,639,242,637" href="http://parts.igem.org/Part:BBa_J23110" target="" /><area shape="poly" alt="" title="" coords="1114,600,1101,631,1189,662,1196,625,1197,625" href="http://parts.igem.org/Part:BBa_K1216000" target="" /><!-- Created by Online Image Map Editor (http://www.maschek.hu/imagemap/index) --></map>
+<script type="text/javascript">
+$(document).ready(function(){
+  $('#imagemap3').mapster({
+    fillColor: 'c2d8f1',
+    fillOpacity: 0.6, /*
+    stroke: true,
+    strokeColor: 'c2d8f1',
+    strokeOpacity: 0.7,
+    strokeWidth: 2, */
+    clickNavigate: true
+  });
+});
+</script>
 </html>

Team:ETH Zurich/Data Page

From 2013.igem.org