Gene circuit

Feel free to click on parts to go to the according registry entry

Our favorite new parts

1. [http://parts.igem.org/Part:BBa_K1216002 Main Page] - Acetyl esterase (Aes) BBa_K1216002: is a hydrolase originated from Escherichia Coli which can be used as a reporter enzyme in synthetic biology. Different butyrate-substrate can be used to detect either a fluorescent or colorimetric signal after cleavage, depending on your requirements. We characterized the enzyme by using the blue 5-Bromo-6-Chloro-3-indoxyl butyrate and the fluorescent 4-Methylumbelliferone. Therefore we did a Michaelis Menten Kinetic of the cell lysate overexpressing Aes (Km=31.47+-12.51 uM).
2. [http://parts.igem.org/Part:BBa_K1216005 MainPage] - Alkaline phosphatase with His tag and TEV cleavage site(phoA), BBa_K1216002 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert 4-Nitrophenylphosphate into an yellow analog precipitate
3. [http://parts.igem.org/Part:BBa_K1216007 MainPage] - pLuxR1 mutated promoter, BBa_K1216007 : the promoter has an EC₅₀ of 6.462 nM in liquid culture. This sensitivity is shifted 300'000 fold compared to the original BBa_R0062 pLuxR. The data were obtained by single cell analysis using a FACS device.

Characterized pre-existing parts :

1. [http://parts.igem.org/Part:BBa_R0062:Experience Experience] - pLuxR wild type, BBa_R0062, Antiquity (2003-01-31) : the promoter has an EC₅₀ of 0.02 nM in liquid culture and 4.45 nM on agar plates. The data were obtained by single cell analysis using a FACS device.
2. [http://parts.igem.org/Part:BBa_J61032:Experience Experience] - Alkaline phosphatase, BBa_J61032, Arkin Lab(2006-11-10) : phoA gene originated from Citrobacter. We did Michealis-Menten kinetics of the phoA enzyme and also showed the conversion of PNP (para-nitrophenol phosphate) in a yellow analog precipitate

Characterized new parts

1. [http://parts.igem.org/Part:BBa_K1216006 Main Page] - Acetyl esterase (AES)with His-Tag and TEV cleavage site, BBa_K1216006 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert into an indigo analog precipitate
2. [http://parts.igem.org/Part:BBa_K1216000 MainPage] - β-Glucuronidase (gusA), BBa_K1216000 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert into an red analog precipitate.
3. [http://parts.igem.org/Part:BBa_K1216004 MainPage] - β-Glucuronidase (gusA)with HIS-Tag and TEV cleavage site, BBa_K1216004 :We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert into an red analog precipitate.
4. [http://parts.igem.org/Part:BBa_K1216003 MainPage] - β-N-Acetylglucosaminidase (nagZ), BBa_K1216003 : We did Michealis-Menten kinetics with cell free extract and showed that the hydrolase can convert into a blue analog precipitate.

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