Team:ETH Zurich/Experiments 4
From 2013.igem.org
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What about the hydolases ? How do they work and where do they come from ? Why do we use hydrolases ? | What about the hydolases ? How do they work and where do they come from ? Why do we use hydrolases ? | ||
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+ | These enzymes have features that make them attractive as reporters. They are known to be relatively stable, exhibit activity under different conditions, and various colorimetric and fluorescent substrates are available. | ||
<h1><b>LacZ : beta-galactosidase</b></h1> | <h1><b>LacZ : beta-galactosidase</b></h1> | ||
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<h1><b>Nagz : Glycoside hydrolase</b></h1> | <h1><b>Nagz : Glycoside hydrolase</b></h1> | ||
<h1><b>GusA : beta-glucuronidase</b></h1> | <h1><b>GusA : beta-glucuronidase</b></h1> | ||
+ | The ''E. coli'' β-glucuronidase is a tetrameric enzyme that hydrolyze of a wide variety of β-glucuronides. |
Revision as of 11:13, 3 September 2013
What about the hydolases ? How do they work and where do they come from ? Why do we use hydrolases ?
These enzymes have features that make them attractive as reporters. They are known to be relatively stable, exhibit activity under different conditions, and various colorimetric and fluorescent substrates are available.
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