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Team:ETH Zurich/Experiments 4
From 2013.igem.org
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<h1><b>Nagz : Glycoside hydrolase</b></h1> | <h1><b>Nagz : Glycoside hydrolase</b></h1> | ||
<h1><b>GusA : beta-glucuronidase</b></h1> | <h1><b>GusA : beta-glucuronidase</b></h1> | ||
| - | The ''E. coli'' β-glucuronidase is a tetrameric enzyme that hydrolyze | + | The ''E. coli'' β-glucuronidase is a tetrameric enzyme that hydrolyze a wide variety of β-glucuronides. |
<br clear="all"/> | <br clear="all"/> | ||
Revision as of 11:21, 3 September 2013
What about the hydolases ? How do they work and where do they come from ? Why do we use hydrolases ?
These enzymes have features that make them attractive as reporters. They are known to be relatively stable, exhibit activity under different conditions, and various colorimetric and fluorescent substrates are available.
Contents |
LacZ : beta-galactosidase
PhoA : Alkaline phosphatase
Aes : Carboxyl esterase
Nagz : Glycoside hydrolase
GusA : beta-glucuronidase
The E. coli β-glucuronidase is a tetrameric enzyme that hydrolyze a wide variety of β-glucuronides.
