Thermo scientific® NanoDrop® 2000 spectrophotometer

In order to measure the concentrations of our plasmids after the Miniprep we used the NanoDrop® 2000 spectrophotometer:

NanoDrop 2000
UV-Vis Spectrophotometer
Smart. Simple. Robust.

The NanoDrop 2000 is a microvolume spectrophotometer for measuring DNA, RNA, and protein. Using the patented sample retention system* the NanoDrop 2000 accurately measures samples as small as 0.5 µL, and reports sample concentration, purity ratios, and full spectral data.

Full spectral microvolume UV-Vis analysis:

Fast measurement time of less than 5 seconds – just pipette, measure, and wipe clean
Measure 0.5 - 2 µL DNA, RNA, and protein samples
Software includes pre-configured methods for measuring nucleic acids, Protein A280 and colorimetric assays, and flexible customized methods
Measure concentrations up to 15,000 ng/µl (dsDNA) - eliminating the need for dilutions
Low-cost operation – no plates or other consumables
Perfect for proteins with low wavelength absorbance, such as peptides at 205 nm

Figure 2. NanoDrop® application for plasmid concentration measurement

Classic NanoDrop Applications

Nucleic Acid
Protein A280
Micro Array
Proteins & Labels
BCA, Bradford, Lowry,
Pierce 660 nm
UV-Vis
Cell Cultures
plus Diagnostics
New Applications

User defined custom methods
New and updated

Intuitive user interface
Flexible reporting & data export
Oligo calculator, units flexibility & more

References

Tecan® Infinite M200 Pro and Tecan iControl

Amersham Biosciences® Novaspec Plus

Biostep® Dark-Hood DH-50 and Argus X1