Thermo scientific® NanoDrop® 2000 spectrophotometer

In order to measure the concentrations of our plasmids after the Miniprep we used the NanoDrop® 2000 spectrophotometer:

NanoDrop 2000
UV-Vis Spectrophotometer
Smart. Simple. Robust.

The NanoDrop 2000 is a microvolume spectrophotometer for measuring DNA, RNA, and protein. Using the patented sample retention system* the NanoDrop 2000 accurately measures samples as small as 0.5 µL, and reports sample concentration, purity ratios, and full spectral data.

Full spectral microvolume UV-Vis analysis:

Fast measurement time of less than 5 seconds – just pipette, measure, and wipe clean
Measure 0.5 - 2 µL DNA, RNA, and protein samples
Software includes pre-configured methods for measuring nucleic acids, Protein A280 and colorimetric assays, and flexible customized methods
Measure concentrations up to 15,000 ng/µl (dsDNA) - eliminating the need for dilutions
Low-cost operation – no plates or other consumables
Perfect for proteins with low wavelength absorbance, such as peptides at 205 nm

Figure 2. NanoDrop® application for plasmid concentration measurement

Classic NanoDrop Applications

Nucleic Acid
Protein A280
Micro Array
Proteins & Labels
BCA, Bradford, Lowry,
Pierce 660 nm
UV-Vis
Cell Cultures
plus Diagnostics
New Applications

User defined custom methods
New and updated

Intuitive user interface
Flexible reporting & data export
Oligo calculator, units flexibility & more

References

Tecan® Infinite M200 Pro and the Tecan iControl software

The fluorescence measurement were perfomed using the Infinite M200 Pro :

Multimode microplate readers for infinite application flexibility and high-level of performance

The Infinite 200 PRO is a user-friendly and affordable multimode reader, designed to cater for the needs of today’s applications. Based on the highly successful Infinite 200 series of microplate reader, the Infinite 200 PRO can provide a full range of leading detection methods in one easy-to-use modular instrument, with either Quad4 monochromator™ or filter-based technologies. Users can select the desired modules to create the perfect reader for their needs, with the option to upgrade as requirements change. The Infinite 200 PRO offers excellent sensitivity, multiplexing capabilities and high format flexibility, including 6- to 384-well microplates, PCR plates, cuvettes and Tecan’s patented NanoQuant Plate™ for low sample volumes.

For uncompromised performance in all detection modes, the Infinite 200 PRO uses advanced optics and high-performance detectors, optimized for the requirements of fluorescence, luminescence and absorbance reading. The Infinite 200 PRO offers a wide range of detection modes, including:

Fluorescence intensity (top and bottom reading) (UV – NIR & wavelength scanning)
Fluorescence resonance energy transfer (FRET)
Time resolved fluorescence energy transfer (TR-FRET) like HTRF®
Time resolved fluorescence (TRF)
Fluorescence polarization (FP)
Flash luminescence
Glow luminescence
Dual-color luminescence (including BRET 1 & BRET 2 applications)
Absorbance (UV – NIR & wavelength scanning)
AlphaScreen® and AlphaLISA® technology
Injectors system for up to two reagents
Temperature control
Small sample measurement in NanoQuant Plate



i-control™ – Microplate Reader Software

The Infinite® series of Tecan readers (Infinite 200, Infinite F500 and Infinite M1000) comes complete with i-control, the easy-to-use microplate reader software. It allows the user to define the workflow for each application. Each workflow is easily created by dragging and dropping the processing steps into a sequence according to the assay protocol. The application workflow is then visible to the user and can be saved for future use. Data are easily managed and exported to Windows® compatible formats (Excel®). Intuitive

Easy set up of workflows via drag & drop
Workflows are visible to the user
Workflows may be saved as measurement scripts
On-line data presentation in Excel®
Graphical definition of measurement range
Plate definition editor
Enhanced data management
Export of data into user-defined Excel® templates
Flexible
User sets up the workflow to suit his own application
Multilabel measurements for multiplexed assays
Different kinds of kinetic measurements
Supports ratio mode also for well kinetic measurements

References

Amersham Biosciences® Novaspec Plus

Novaspec Plus Visible Spectrophotometer

Novaspec™ Plus Visible Spectrophotometer is ideal for general biotech laboratory use. There are stored methods for the standard colorimetric methods of Bradford, BCA, Biuret and Lowry in addition to the basic modes of absorbance, transmittance,OD600 and concentration measurements. The use of diode array technology permits rapid wavelength scans to be made as well and, as there are no moving parts, make for a highly reliable and low maintenance product (Fig 1).
The large backlit display enables the graphical results for wavelength scans, kinetics assays (including slope calculation for rate/activity studies) and standard curves to be viewed (Fig 2).Up to 99 methods for such experiments can be stored for instant recall and use by a laboratory assistant. Novaspec Plusis delivered with Grafico, a PC utility software package, and the requisite serial lead, providing the user with the means to capture, print and store data from the instrument (with a time/date stamp) onto a PC so that a results log can be built up or data exported to Microsoft™ Excel.

The cell holder supplied with Novaspec Plus accepts standard 10-mm pathlength glass or plastic cells (adapters are availableto convert it to accept 10-, 12- and 16-mm diameter test tubes).It can be removed for cleaning, or flushed through with water in situ, if spillages occur.
For users who require thermostatting, particularly for kineticsstudies and use with test kits, a special version of Novaspec Plus is available with a factory fitted heated cell holder (37 °C only);note that this cell holder cannot be added retrospectively.

Features

“Flash Scan” diode array
Stored protein methods
Bacterial cell culture measurement at OD600
Kinetics for activity studies
Stored methods

References

Biostep® Dark-Hood DH-50 and the Argus X1 software

Dark Hood DH-50 with control panel and display

with sliding table, UV protection shield, CCD
stationary dark hood with large door
sliding table to put on a transilluminator* incl. UV protection shield – no additional protection necessary
special bracket for CCD camera
footprint (WxD) 55x48cm
height incl. camera bracket: 88cm
max. filter size of transilluminator 30x23cm
dimmable white top-light by LEDs
automatic UV cut-off when opening the UV protection shield
preparative function enables observation of gels under UV light
control panel with keypad and LCD display
3 USB ports

Functionality:

transilluminator: on/off
white top-light: on/off, dim function
preparative function
optionally available: control of zoom, aperture and focus by motor zoom objective
optionally available: storage of application methods repeatable
transilluminators with dimensions (W x D x H): 32.5 x 32.2 x 10.5cm; corresponds to the series of biostep UST, USDT, UXFT, BST, GST, YST, RST, WST

Optionally upgradeable with UV protection shield, filter wheel, white transmission light, UV or epi light in the visible range

References

biostep argusX1® basic licence

PC-software for controlling the camera basic modules Felix 1000/2000, 5000/6000/7000 and the transmission scanners of the series ViewPix 900/1300

Attention:
Imperatively necessary: module for controlling the camera or scanner!!!

General software functions:
integrated database for administration of the images
copying and cropping of single images or group images within a database or even between database to database if the additional module Project administration exists
deletion of single images or group images
functions for image processing incl. Undo
rotating in arbitrary angles and around 90°, 180°
reflecting in horizontal and vertical direction
inverting
cropping
various filter functions
marking of the images with text, rectangle and line
automated generation and numeration of sample names after the acquisition
memo function: input of a note for each image and printout of the same on a protocol
history for documentation of the acquisition parameters for each image
overexposure control
data export into other Windows applications
data import of images in different formats
creation of individual print reports
quick print
selection of the export format, export path, print report for quick print
selection of the user language: German, English, Spanish, French or Italian
control of the functions a of biostep transilluminators with PC interface

References