Thermo scientific® NanoDrop® 2000 spectrophotometer

In order to measure the concentrations of our plasmids after the Miniprep we used the NanoDrop® 2000 spectrophotometer:

NanoDrop 2000
UV-Vis Spectrophotometer
Smart. Simple. Robust.

The NanoDrop 2000 is a microvolume spectrophotometer for measuring DNA, RNA, and protein. Using the patented sample retention system* the NanoDrop 2000 accurately measures samples as small as 0.5 µL, and reports sample concentration, purity ratios, and full spectral data.

Full spectral microvolume UV-Vis analysis:

Fast measurement time of less than 5 seconds – just pipette, measure, and wipe clean
Measure 0.5 - 2 µL DNA, RNA, and protein samples
Software includes pre-configured methods for measuring nucleic acids, Protein A280 and colorimetric assays, and flexible customized methods
Measure concentrations up to 15,000 ng/µl (dsDNA) - eliminating the need for dilutions
Low-cost operation – no plates or other consumables
Perfect for proteins with low wavelength absorbance, such as peptides at 205 nm

Figure 2. NanoDrop® application for plasmid concentration measurement

Classic NanoDrop Applications

Nucleic Acid
Protein A280
Micro Array
Proteins & Labels
BCA, Bradford, Lowry,
Pierce 660 nm
UV-Vis
Cell Cultures
plus Diagnostics
New Applications

User defined custom methods
New and updated

Intuitive user interface
Flexible reporting & data export
Oligo calculator, units flexibility & more

References

Tecan® Infinite M200 Pro and the Tecan iControl software

The fluorescence measurement were perfomed using the Infinite M200 Pro :

Multimode microplate readers for infinite application flexibility and high-level of performance

The Infinite 200 PRO is a user-friendly and affordable multimode reader, designed to cater for the needs of today’s applications. Based on the highly successful Infinite 200 series of microplate reader, the Infinite 200 PRO can provide a full range of leading detection methods in one easy-to-use modular instrument, with either Quad4 monochromator™ or filter-based technologies. Users can select the desired modules to create the perfect reader for their needs, with the option to upgrade as requirements change. The Infinite 200 PRO offers excellent sensitivity, multiplexing capabilities and high format flexibility, including 6- to 384-well microplates, PCR plates, cuvettes and Tecan’s patented NanoQuant Plate™ for low sample volumes.

For uncompromised performance in all detection modes, the Infinite 200 PRO uses advanced optics and high-performance detectors, optimized for the requirements of fluorescence, luminescence and absorbance reading. The Infinite 200 PRO offers a wide range of detection modes, including:

Fluorescence intensity (top and bottom reading) (UV – NIR & wavelength scanning)
Fluorescence resonance energy transfer (FRET)
Time resolved fluorescence energy transfer (TR-FRET) like HTRF®
Time resolved fluorescence (TRF)
Fluorescence polarization (FP)
Flash luminescence
Glow luminescence
Dual-color luminescence (including BRET 1 & BRET 2 applications)
Absorbance (UV – NIR & wavelength scanning)
AlphaScreen® and AlphaLISA® technology
Injectors system for up to two reagents
Temperature control
Small sample measurement in NanoQuant Plate



i-control™ – Microplate Reader Software

The Infinite® series of Tecan readers (Infinite 200, Infinite F500 and Infinite M1000) comes complete with i-control, the easy-to-use microplate reader software. It allows the user to define the workflow for each application. Each workflow is easily created by dragging and dropping the processing steps into a sequence according to the assay protocol. The application workflow is then visible to the user and can be saved for future use. Data are easily managed and exported to Windows® compatible formats (Excel®). Intuitive

Easy set up of workflows via drag & drop
Workflows are visible to the user
Workflows may be saved as measurement scripts
On-line data presentation in Excel®
Graphical definition of measurement range
Plate definition editor
Enhanced data management
Export of data into user-defined Excel® templates
Flexible
User sets up the workflow to suit his own application
Multilabel measurements for multiplexed assays
Different kinds of kinetic measurements
Supports ratio mode also for well kinetic measurements
References

Amersham Biosciences® Novaspec Plus

Biostep® Dark-Hood DH-50 and Argus X1