Team:ETH Zurich/Modeling

From 2013.igem.org

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<b>Mine Cells</b>
<b>Mine Cells</b>
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[[File:eqnSender.png|500px|thumb|Equation system 1: ODE for Mine Cells.]]
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The Mine Cells lead to the synthesis the signalling molecule, by constitutive expression of ''luxI'' gene. To reveal the nature of the cells, a coloured-substrate reaction is triggered upon addition of ''5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide''; given that the glycoside hydrolase ''NagZ'' is expressed constitutively. <br><br> The ODEs for the states involved in the sender module are given below:
The Mine Cells lead to the synthesis the signalling molecule, by constitutive expression of ''luxI'' gene. To reveal the nature of the cells, a coloured-substrate reaction is triggered upon addition of ''5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide''; given that the glycoside hydrolase ''NagZ'' is expressed constitutively. <br><br> The ODEs for the states involved in the sender module are given below:
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<b>Biosensors</b>
<b>Biosensors</b>
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[[File:eqnBiosensor.png|500px|thumb|Equation system 1: ODE for Mine Cells.]]
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[[File:eqnBiosensor.png|500px|right]]
Our Biosensor cells are engineered to respond differently to high and medium concentrations of AHL. This cells are capable of discriminate between the presence of 1 or 2 mine cells around them in the immediate vicinity. To accomplish this task, the enzymes involved in the coloured-substrate reaction are sensitive to different concentrations of the dimer LuxR-AHL (denoted as R).
Our Biosensor cells are engineered to respond differently to high and medium concentrations of AHL. This cells are capable of discriminate between the presence of 1 or 2 mine cells around them in the immediate vicinity. To accomplish this task, the enzymes involved in the coloured-substrate reaction are sensitive to different concentrations of the dimer LuxR-AHL (denoted as R).
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Revision as of 12:31, 19 August 2013

Header2.png
80px-Eth igem logo.png


The digital bacterial-based minesweeper

The change of the species concentrations in time is given by non-linear ordinary differential equations (ODEs), most of which follow Hill kinetics. The parameters we used in the model are derived from literature. In our model we consider that signalling molecules degrade at the same rate whether they are cytoplasmic or not.


Mine Cells

EqnSender.png

The Mine Cells lead to the synthesis the signalling molecule, by constitutive expression of luxI gene. To reveal the nature of the cells, a coloured-substrate reaction is triggered upon addition of 5-Bromo-4-chloro-3-indoxyl-N-acetyl-beta-D-glucosaminide; given that the glycoside hydrolase NagZ is expressed constitutively.

The ODEs for the states involved in the sender module are given below:


Biosensors

EqnBiosensor.png

Our Biosensor cells are engineered to respond differently to high and medium concentrations of AHL. This cells are capable of discriminate between the presence of 1 or 2 mine cells around them in the immediate vicinity. To accomplish this task, the enzymes involved in the coloured-substrate reaction are sensitive to different concentrations of the dimer LuxR-AHL (denoted as R).