Team:Evry

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<a href='https://2013.igem.org/Team:Evry/Home' title='Vers la page française'> <img src='https://static.igem.org/mediawiki/2013/b/b9/Francais.jpg'/></a>
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<h1 align='center'>Welcome to the Iron coli Project</h1>
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<h1>Welcome to the <b><span style="color:#bb8900">Iron Coli Project</span></b> Home Page</h1>
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<p align="center"><b>The aim of our team this year is to design and realize a <em>treatment for hemochromatosis and thalassemia</em> using synthetic biology with an <em>integrated human practice</em> and <em>modeling approach</em>. </b></p>
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<h3 align="center">The Team</h3>
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<h3 align="center">Abstract</h3>
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<h2 align='center'>Abstract</h2>
 
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Our starting idea was a very simple disease: hemochromatosis. The consequences are chronic insufficiencies due to an iron overload in blood. The only working treatment was regular bloodletting and this appeared as a very archaic technique which contrasted with the actual advances in the medical field. After deeper research on the disease, we figured out that the symptoms of the disease are the consequence of an iron overabsorption from the intestins. At this very moment, we came to the idea to design a bacterium able to chelate iron in the intestins. The challenge initially is great, and seemed unfeasable. However, after finding out that Escherichia coli naturally produced siderophores, we then chose to make it
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Our project focuses on developing a novel treatment for <a href="https://2013.igem.org/Team:Evry/Project_diseases"><em>hematological disorders</em></a>  caused by an <a href="https://2013.igem.org/Team:Evry/Project_metabolism"><em>iron overload</em></a>, such as hemochromatosis and thalassemia. These autosomal recessive disorders have symptoms including cirrhosis, arthritis, and heart failure, which result from overabsorption of iron from the duodenum. Although these are among the most common heritable diseases, treatment options are limited. Even today patients are mostly treated by frequent bloodletting, which <a href="https://2013.igem.org/Team:Evry/HumanPractice/Patients_perceptions"><em>many people cannot support</em></a>. The aim of our project is to combat these diseases at the source by developing a therapy that prevents the intestinal absorption of iron.
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Bloodletting consists of the withdrawal of blood for therapeutic purposes. It is one of the oldest medical techniques and is nowadays abandonned. However in hematology, bloodletting (also known as phlebotomy) seems to remain the only reliable solution to cure hemochromatosis and thalassemia. Both are genetic diseases diseases and consist of an iron overload. The iron ion is toxic due to its oxidative potential, and thus, has a very smooth regulation mechanism. In fact, the organism only absorbs the required amount of iron from the intestins. The pathogenic mechanism is due to a seemingly low iron rate in the organism and permanantly activate the absorption system by the instestins, thus leading to an iron overload. The consequences are mainly chronic insufficiencies (renal, hepatic, cardiac, ...). Despite the true efficiency of bloodletting, some patients cannot bear this archaic way of treating their disease. As a consequence, we decided to design an Escherichia coli capable of chelating iron in the intestins: Iron Coli. The bacterium will lower the amount of iron absorbed by the duodenum and reduce the frequency of bloodlettings. Also, certain patients are treated with iron chelators which have a lot of side effects. The reduction of iron absorption by Iron Coli will allow lower drug concentrations and improve the patient's comfort.
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We engineer the <i>Escherichia coli</i> <a href="https://2013.igem.org/Team:Evry/Project_FUR"><em>Ferric Uptake Regulation</em></a> (FUR) system using a <a href="https://2013.igem.org/Team:Evry/Inverter"><em>genetic inverter</em></a> so that they produce siderophores (<a href="https://2013.igem.org/Team:Evry/Chelator"><em>iron chelators</em></a>) in response of high concentrations of iron. These engineered bacteria are delivered to the patient's intestine by encapsulating them in an ingestible polymer (<a href="https://2013.igem.org/Team:Evry/Pill_design"><em>capsule</em></a>)  that specifically degrades in the duodenum. Once released into the intestine, the bacteria respond to ambient iron by secreting elevated levels of siderophores, thereby chelating the iron to prevent its absorption by the patient.
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<h3 align="center">Overview of the project and the achievements</h3>
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alert("Our team is proud to announce that we were awarded a gold medal and the Best Human Practice price at the european jamboree! We are going to Boston.\n\nUnfortunately, we are missing a few thousands of euros to be capable of finishing the competition. If you are a company and would like to sponsor our team, please contact us at igemevry@gmail.com.")
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Latest revision as of 18:38, 28 October 2013

Iron coli project

Welcome to the Iron Coli Project Home Page


The aim of our team this year is to design and realize a treatment for hemochromatosis and thalassemia using synthetic biology with an integrated human practice and modeling approach.



The Team

team photo

Abstract

Our project focuses on developing a novel treatment for hematological disorders caused by an iron overload, such as hemochromatosis and thalassemia. These autosomal recessive disorders have symptoms including cirrhosis, arthritis, and heart failure, which result from overabsorption of iron from the duodenum. Although these are among the most common heritable diseases, treatment options are limited. Even today patients are mostly treated by frequent bloodletting, which many people cannot support. The aim of our project is to combat these diseases at the source by developing a therapy that prevents the intestinal absorption of iron.

We engineer the Escherichia coli Ferric Uptake Regulation (FUR) system using a genetic inverter so that they produce siderophores (iron chelators) in response of high concentrations of iron. These engineered bacteria are delivered to the patient's intestine by encapsulating them in an ingestible polymer (capsule) that specifically degrades in the duodenum. Once released into the intestine, the bacteria respond to ambient iron by secreting elevated levels of siderophores, thereby chelating the iron to prevent its absorption by the patient.

Overview of the project and the achievements