Team:Evry

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<h1>Welcome to the <b><span style="color:#bb8900">Iron Coli Project</span></b> Home Page</h1>
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<p align="center"><b>The aim of our team this year is to design and realize a <em>treatment for hemochromatosis and thalassemia</em> using synthetic biology with an <em>integrated human practice</em> and <em>modeling approach</em>. </b></p>
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<h1>Welcome to the Iron coli Project Home Page</h1>
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<h3 align="center">The Team</h3>
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<h3>Authors</h3>
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<h3 align="center">Abstract</h3>
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E. van der Kouwe<sup>1, 2</sup>, G. Mercy<sup>3</sup>, B. Baudu<sup>4</sup>, A. Chhun<sup>1</sup>, F. Amiot<sup>1</sup>, G. Guillocheau<sup>1</sup>, L. Le Goff<sup>5</sup>, H. Léger<sup>5</sup>, L. Ujéda<sup>6, 11</sup>, W. Rostain<sup>7</sup>, C. Pauthenier<sup>8</sup>, T. Cerisy<sup>9</sup>, P. Parutto<sup>8</sup>, A. Jaramillo<sup>7</sup>, A. Tolonen<sup>9</sup> and N. Pollet<sup>10</sup>
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Our project focuses on developing a novel treatment for <a href="https://2013.igem.org/Team:Evry/Project_diseases"><em>hematological disorders</em></a> caused by an <a href="https://2013.igem.org/Team:Evry/Project_metabolism"><em>iron overload</em></a>, such as hemochromatosis and thalassemia. These autosomal recessive disorders have symptoms including cirrhosis, arthritis, and heart failure, which result from overabsorption of iron from the duodenum. Although these are among the most common heritable diseases, treatment options are limited. Even today patients are mostly treated by frequent bloodletting, which <a href="https://2013.igem.org/Team:Evry/HumanPractice/Patients_perceptions"><em>many people cannot support</em></a>. The aim of our project is to combat these diseases at the source by developing a therapy that prevents the intestinal absorption of iron.
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<h3>Abstract</h3>
 
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This year, our project focuses on hematological disorders. More specifically, we focus on diseases that are subsequent to an iron overload such as hemochromatosis and thalassemia. For hereditary hemochromatosis, the symptoms are due to the overabsorption of iron from the duodenum by the mutation of the HFE protein. However, in case of thalassemia, patients excessively absorb iron because of a subsequent metabolic perturbation.
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We engineer the <i>Escherichia coli</i> <a href="https://2013.igem.org/Team:Evry/Project_FUR"><em>Ferric Uptake Regulation</em></a> (FUR) system using a <a href="https://2013.igem.org/Team:Evry/Inverter"><em>genetic inverter</em></a> so that they produce siderophores (<a href="https://2013.igem.org/Team:Evry/Chelator"><em>iron chelators</em></a>) in response of high concentrations of iron. These engineered bacteria are delivered to the patient's intestine by encapsulating them in an ingestible polymer (<a href="https://2013.igem.org/Team:Evry/Pill_design"><em>capsule</em></a>)  that specifically degrades in the duodenum. Once released into the intestine, the bacteria respond to ambient iron by secreting elevated levels of siderophores, thereby chelating the iron to prevent its absorption by the patient.
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Nowadays, the iron overload is mainly treated by bloodlettings for hemochromatosic patients but cannot be extended to thalassemic patients who suffer from anaemia. The aim of our project is to prevent the intestinal absorption of iron, thus acting directly at the source.
 
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Using the Ferric Uptake Regulation (FUR) system that controls siderophore biosynthesis (iron chelator), we engineer <i>Escherichia coli</i> in order to produce these siderophores in response of high concentrations of iron. To reduce the patient's iron absorption, our bacteria will be placed in a capsule and will be ingested during a meal. Once it will arrive in the duodenum, our bacteria will produce the siderophore at their full potential and chelate the iron.
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<h3>Affiliations</h3>
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<h3 align="center">Overview of the project and the achievements</h3>
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<sup>1</sup>Université Evry Val d'Essonne
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<sup>2</sup>Faculté de Pharmacie de Lyon
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<sup>3</sup>Sup'Biotech Paris
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<sup>4</sup>AgroParisTech
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<sup>5</sup>Ecole Nationale Supérieure d'Informatique pour l'Industrie et l'Entreprise
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<sup>6</sup>Sciences Po Paris
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<sup>7</sup>Synth Bio Team, Institute of Systems and Synthetic Biology
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<sup>8</sup>Bio-RetroSynth Team, Institute of Systems and Synthetic Biology
 
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<sup>9</sup>LGBM, Genoscope
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<sup>10</sup>Metamorphosys, Institute of Systems and Synthetic Biology
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alert("Our team is proud to announce that we were awarded a gold medal and the Best Human Practice price at the european jamboree! We are going to Boston.\n\nUnfortunately, we are missing a few thousands of euros to be capable of finishing the competition. If you are a company and would like to sponsor our team, please contact us at igemevry@gmail.com.")
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Latest revision as of 18:38, 28 October 2013

Iron coli project

Welcome to the Iron Coli Project Home Page


The aim of our team this year is to design and realize a treatment for hemochromatosis and thalassemia using synthetic biology with an integrated human practice and modeling approach.



The Team

team photo

Abstract

Our project focuses on developing a novel treatment for hematological disorders caused by an iron overload, such as hemochromatosis and thalassemia. These autosomal recessive disorders have symptoms including cirrhosis, arthritis, and heart failure, which result from overabsorption of iron from the duodenum. Although these are among the most common heritable diseases, treatment options are limited. Even today patients are mostly treated by frequent bloodletting, which many people cannot support. The aim of our project is to combat these diseases at the source by developing a therapy that prevents the intestinal absorption of iron.

We engineer the Escherichia coli Ferric Uptake Regulation (FUR) system using a genetic inverter so that they produce siderophores (iron chelators) in response of high concentrations of iron. These engineered bacteria are delivered to the patient's intestine by encapsulating them in an ingestible polymer (capsule) that specifically degrades in the duodenum. Once released into the intestine, the bacteria respond to ambient iron by secreting elevated levels of siderophores, thereby chelating the iron to prevent its absorption by the patient.

Overview of the project and the achievements

Retrieved from "http://2013.igem.org/Team:Evry"