Revision as of 08:48, 9 August 2013

Iron coli project

Welcome to the Iron coli Project

Abstract

This year, our project focuses on hematological disorders. More specifically, we focus on diseases that are subsequent to an iron overload such as hemochromatosis and thalassemia. For hereditary hemochromatosis, the symptoms are due to the overabsorption of iron from the duodenum by the mutation of the HFE protein. However, in case of thalassemia, patients excessively absorb iron because of a subsequent metabolic perturbation.

Nowadays, the iron overload is mainly treated by bloodlettings for hemochromatosic patients but cannot be extended to thalassemic patients who suffer from anaemia. Our project focuses on preventing the intestinal absorption of iron, thus acting directly at the source.

Using the Ferric Uptake Regulation (FUR) system that controls siderophore biosynthesis (iron chelator), we engineer Escherichia coli in order to produce these siderophores in response of high concentrations of iron. To reduce the patient's iron absorption, our bacteria will be placed in a capsule and will be ingested during a meal. Once it will arrive in the duodenum, our bacteria will produce the siderophore at their full potential and chelate the iron.

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 <h1 align='center'>Welcome to the Iron coli Project</h1>
 <h2 align='center'>Abstract</h2>
-<p>
-This year we choose to develop a project which deals with hematologic diseases. We focused specifically on diseases with an iron overload disorder. Among those type of diseases, we were interested in the hereditary hemochromatosis and hemosiderosis. In the case of hereditary hemochromatosis, the iron overload disorder is a direct consequence of this disease. While in the hemosiderosis the iron overload is due to a metabolic perturbation.
-</p>
-<p>
-Today the iron overload can be treated by bloodletting in the case of the hemochromatosis but it cannot be used as a treatment of hemosiderosis because patients suffer of anaemia. In our project we want to solve this problem acting directly at the iron absorption source, in the intestines.
-</p>
-<p>
-Using the Ferric Uptake Regulation (FUR) system to control siderophore (iron chelator) biosynthesis, we engineered bacteria giving them the ability to produce these siderophores in response of iron. In order to limit the iron overload in the patient, these bacteria will be placed in capsule and ingested during a meal. Arriving in the duodenum our bacteria will limit iron absorption by chelating it.
-</p>
-<h2 align='center'>Abstract vE</h2>
 <p>
 This year, our project focuses on hematological disorders. More specifically, we focus on diseases that are subsequent to an iron overload such as hemochromatosis and thalassemia. For hereditary hemochromatosis, the symptoms are due to the overabsorption of iron from the duodenum by the mutation of the HFE protein. However, in case of thalassemia, patients excessively absorb iron because of a subsequent metabolic perturbation.
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 <p>
-Using the Ferric Uptake Regulation (FUR) system that controls siderophore biosynthesis (iron chelator), we engineer Escherichia coli in order to produce these siderophores in response of high concentrations of iron. To reduce the patient's iron absorption, our bacteria is placed in a capsule and will be ingested during a meal. Once arrived in the duodenum, our bacteria produce the siderophore at their full potential and chelate the iron.
+Using the Ferric Uptake Regulation (FUR) system that controls siderophore biosynthesis (iron chelator), we engineer <i>Escherichia coli</i> in order to produce these siderophores in response of high concentrations of iron. To reduce the patient's iron absorption, our bacteria will be placed in a capsule and will be ingested during a meal. Once it will arrive in the duodenum, our bacteria will produce the siderophore at their full potential and chelate the iron.
 </p>