Team:Freiburg/Highlights
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- | <p>We developed a novel and innovative ELISA-based method to quantify the binding efficiencies of our dCas9 fusion proteins: The uniCAS Binding Assay (uniBAss). Biotinylated oligos are coated on 96-well ELISA plates via the interaction with streptavidin and bound dCas9/RNA complexes can be detected via antibodies. We were able to show that | + | <p>We developed a novel and innovative ELISA-based method to quantify the binding efficiencies of our dCas9 fusion proteins: The uniCAS Binding Assay (uniBAss). Biotinylated oligos are coated on 96-well ELISA plates via the interaction with streptavidin and bound dCas9/RNA complexes can be detected via antibodies. We were able to show that this is a powerful tool to characterize the modified dCas9 fusion proteins by assessing their DNA binding capacity with possible improvements for high-throughput screenings. <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/method">Read more!</a> |
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Revision as of 09:52, 19 October 2013
HIGHLIGHTS