Team:Freiburg/Project/unibox

From 2013.igem.org

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What you need
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Using the light box and performing light experiments is easy and can be done in every lab. All you need for your experiments is the following:
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<li> The uniBOX (construction see above) </li>
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<li> A common box to keep the samples in dark </li>
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<li> A room that can be made impervious to light (even a storeroom would serve perfectly well) </li>
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<li> An incubator (also impervious to light in the optimal case) </li>
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<li> 2 LED light chains (one for the light box and one for the "dark" roam to get your save light conditions) </li>
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</ul>
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Principals of light experiments
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In principle every light experiment works similar. All you have to do is to proceed your samples under safe light in the dark room. Otherwise it is indispensable to keep your samples in the uniBOX or dark box  to protect them from sunlight and artificial light. Even inside of the incubator this protection is important.  Safe light are light conditions that do not affect your light experiments. That means if you are doing a red light experiment it is important that the safelight do not include wavelengths form 600 to 700 nm. Therefore the safe light conditions for red light can be green light or blue light.  As green light contains less energy it is probably the best choice. Nearly every common green LED can be used to produce safe green light. 
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Revision as of 19:40, 26 October 2013


uniBOX - A lightbox build from lego!

Idea

Construction

Usage

What you need

Using the light box and performing light experiments is easy and can be done in every lab. All you need for your experiments is the following:

  • The uniBOX (construction see above)
  • A common box to keep the samples in dark
  • A room that can be made impervious to light (even a storeroom would serve perfectly well)
  • An incubator (also impervious to light in the optimal case)
  • 2 LED light chains (one for the light box and one for the "dark" roam to get your save light conditions)

Principals of light experiments

In principle every light experiment works similar. All you have to do is to proceed your samples under safe light in the dark room. Otherwise it is indispensable to keep your samples in the uniBOX or dark box to protect them from sunlight and artificial light. Even inside of the incubator this protection is important. Safe light are light conditions that do not affect your light experiments. That means if you are doing a red light experiment it is important that the safelight do not include wavelengths form 600 to 700 nm. Therefore the safe light conditions for red light can be green light or blue light. As green light contains less energy it is probably the best choice. Nearly every common green LED can be used to produce safe green light.

Results

Optimized Protocol