Team:Freiburg/Project/unibox
From 2013.igem.org
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<div id="left_column"> <p id="headline"> <a href="http://parts.igem.org/Part:BBa_K1150000"><img src="https://static.igem.org/mediawiki/2013/9/90/Freiburg-2013-Highlights-Heart.png" style="width:65px; margin-top:-17px; margin-left:-4px; "> </a> | <div id="left_column"> <p id="headline"> <a href="http://parts.igem.org/Part:BBa_K1150000"><img src="https://static.igem.org/mediawiki/2013/9/90/Freiburg-2013-Highlights-Heart.png" style="width:65px; margin-top:-17px; margin-left:-4px; "> </a> | ||
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<p>The CRISPR/Cas9 system relies on a protein-RNA-DNA interaction between the Cas9 protein, two non-coding RNAs and the appropriate DNA. The 160 kDa Cas9 protein was <a id="link" href="https://2013.igem.org/Team:Freiburg/Notebook/standardisation#august">mutated</a> to inactivate the DNA cleavage site. <i>Read more in the next slides</i>.</p> | <p>The CRISPR/Cas9 system relies on a protein-RNA-DNA interaction between the Cas9 protein, two non-coding RNAs and the appropriate DNA. The 160 kDa Cas9 protein was <a id="link" href="https://2013.igem.org/Team:Freiburg/Notebook/standardisation#august">mutated</a> to inactivate the DNA cleavage site. <i>Read more in the next slides</i>.</p> | ||
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- | <a href="http://parts.igem.org/Part:BBa_K1150000"><img src="https://static.igem.org/mediawiki/2013/ | + | <a href="http://parts.igem.org/Part:BBa_K1150000"><img src="https://static.igem.org/mediawiki/2013/c/c8/IMG_4508_500-Freiburg-2013.JPG" style="width:500px; margin-left:5px;"> </a> |
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<div class="frontpage-slide" style="margin-top:-11px;"> | <div class="frontpage-slide" style="margin-top:-11px;"> | ||
<div id="left_column"> <p id="headline"> <a href="http://parts.igem.org/Part:BBa_K1150000"><img src="https://static.igem.org/mediawiki/2013/9/90/Freiburg-2013-Highlights-Heart.png" style="width:65px; margin-top:-17px; margin-left:-4px; "> </a> | <div id="left_column"> <p id="headline"> <a href="http://parts.igem.org/Part:BBa_K1150000"><img src="https://static.igem.org/mediawiki/2013/9/90/Freiburg-2013-Highlights-Heart.png" style="width:65px; margin-top:-17px; margin-left:-4px; "> </a> | ||
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</p> | </p> | ||
<p>The CRISPR/Cas9 system relies on a protein-RNA-DNA interaction between the Cas9 protein, two non-coding RNAs and the appropriate DNA. The 160 kDa Cas9 protein was <a id="link" href="https://2013.igem.org/Team:Freiburg/Notebook/standardisation#august">mutated</a> to inactivate the DNA cleavage site. <i>Read more in the next slides</i>.</p> | <p>The CRISPR/Cas9 system relies on a protein-RNA-DNA interaction between the Cas9 protein, two non-coding RNAs and the appropriate DNA. The 160 kDa Cas9 protein was <a id="link" href="https://2013.igem.org/Team:Freiburg/Notebook/standardisation#august">mutated</a> to inactivate the DNA cleavage site. <i>Read more in the next slides</i>.</p> | ||
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<div id="right_column"> <div style="text-align: center;"> | <div id="right_column"> <div style="text-align: center;"> | ||
- | <a href="http://parts.igem.org/Part:BBa_K1150000"><img src="https://static.igem.org/mediawiki/2013/ | + | <a href="http://parts.igem.org/Part:BBa_K1150000"><img src="https://static.igem.org/mediawiki/2013/e/ea/IMG_4511_500-Freiburg-2013.JPG" style="width:500px; margin-left:5px;"> </a> |
</div> | </div> |
Revision as of 19:37, 27 October 2013
uniBOX - A lightbox build from lego!
Idea
Light inducible systems are more and more used in iGEM and synthetic biology in general. Team Munich for example uses a light inducible killswitch, while Shanghai uses light-controlled expression systems. Even our uniCAS gene regulation system is controllable via light. Therefore we thought of a easy and cheap way to build a light box to illuminate our cells with a certain wavelength. But construction of such a light box can be hard because there are certain problems you fave to face like dimout but still enough oxygen supply or illumination with the right wavelength. Our so called uniBOX can be build by using common things like Lego bricks, glass, foil and LEDs. We were able to show that light systems can be controlled efficiently and we now provide a do-it-yourself manual for building your own uniBOX for less than 60 €!
Construction