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Team:INSA Toulouse/contenu/lab practice/notebook/calendar/logic gates
From 2013.igem.org
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Verfication of the cloning, AND2.RFPinv ok<br><br> | Verfication of the cloning, AND2.RFPinv ok<br><br> | ||
</li> | </li> | ||
| - | <li><a href="">Week | + | <li><a href="">Week 2 (09-15 Sept)</a></li></span><br> |
10/09<br> | 10/09<br> | ||
Cloning AND2.RFPinv with PolT7 and XOR2 with RFPinv<br><br> | Cloning AND2.RFPinv with PolT7 and XOR2 with RFPinv<br><br> | ||
Revision as of 12:30, 30 September 2013
Calendar
Logic Gates Characterization
July 2013
- Week 6 (15-21 July)
15/07
PCR to invert RFP and rbs.LuxI with the restriction site ClaI BamHI
16/07
Purification of the PCR and verification by gel.
19/07
Assembling of rbs.LuxI and cI for the final system with the AND1 gate.
Arrival of the synthesis.
20/07
Verification of the clonage.
Assembling the synthesis parts (the 4 gates) into pSB1C3.
- Week 7 (22-28 July)
22/07
Assembling XOR1 with FimE, LuxI with cI
September 2013
- Week 1 (02-08 Sept)
- Week 2 (09-15 Sept)
06/09
Cloning AND2 and XOR2 with RFPinv
08/09
Verfication of the cloning, AND2.RFPinv ok
10/09
Cloning AND2.RFPinv with PolT7 and XOR2 with RFPinv
11/09
In fusion to have XOR2 with RFPinv because cloning is not working
13/09
XOR2.RFPinv with in fusion OK
14/09
Cloning XOR2.RFPinv and AND2.RFPinv with the good PolT7 (with promoter)
15/09
XOR2.RFPinv and AND2.RFPinv with PolT7 ok for characterisation
