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Team:INSA Toulouse/contenu/project/biological construction/riboregulation
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<img src="https://static.igem.org/mediawiki/2013/1/10/How_does_it_work.png" class="imgcontent" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/How_does_it_work.png" class="imgcontent" /> | ||
| - | <p class="texte">The | + | <p class="texte">The blue and the red regions are regions which code for little RNA sequences. |
<br> | <br> | ||
<br> | <br> | ||
<br> | <br> | ||
| - | When P2 is activated and not P1, the | + | When P2 is activated and not P1, the red sequence will be transcripted. |
| - | This | + | This red sequence possess a similarity with the rbs sequence. So when transcripted the red sequence will confine the rbs and prevent the recombinase from being translated. |
</p> | </p> | ||
<img src="https://static.igem.org/mediawiki/2013/4/48/P2_activated.png" class="imgcontent" /> | <img src="https://static.igem.org/mediawiki/2013/4/48/P2_activated.png" class="imgcontent" /> | ||
| - | <p class="texte">When P1 and P2 are activated, both sequences (the | + | <p class="texte">When P1 and P2 are activated, both sequences (the blue and the red one) are transcripted. |
| - | <br>The | + | <br>The blue RNA sequence possess a stronger similiraty with the red one. When transcripted the blue and the red sequences will match together and release the rbs to permit the translation of the recombinase</p> |
<img src="https://static.igem.org/mediawiki/2013/1/16/P1_and_P2_activated.png" class="imgcontent" /> | <img src="https://static.igem.org/mediawiki/2013/1/16/P1_and_P2_activated.png" class="imgcontent" /> | ||
Revision as of 15:50, 20 August 2013
Biological Construction
Riboregulation System
In order to better control the expression of the recombinases, a ribo regulation system have been add before the recombinases genes.
How does it work ?
This is the basic system : Two promoters each followed by a RNA sequence
The blue and the red regions are regions which code for little RNA sequences.
When P2 is activated and not P1, the red sequence will be transcripted.
This red sequence possess a similarity with the rbs sequence. So when transcripted the red sequence will confine the rbs and prevent the recombinase from being translated.
When P1 and P2 are activated, both sequences (the blue and the red one) are transcripted.
The blue RNA sequence possess a stronger similiraty with the red one. When transcripted the blue and the red sequences will match together and release the rbs to permit the translation of the recombinase
