Team:INSA Toulouse/contenu/project/overview

From 2013.igem.org

(Difference between revisions)
 
(29 intermediate revisions not shown)
Line 8: Line 8:
<html>
<html>
-
  <!--/* open Sans : fonnt Google*/-->
+
  <!--/* open Sans : font Google*/-->
   <link href='http://fonts.googleapis.com/css?family=Open+Sans' rel='stylesheet' type='text/css'>
   <link href='http://fonts.googleapis.com/css?family=Open+Sans' rel='stylesheet' type='text/css'>
Line 26: Line 26:
   .title3{color:#7f8c8c; font-family:'Open Sans'; font-weight:400; font-size:16px; margin:0 0 20px 0; border:none;}
   .title3{color:#7f8c8c; font-family:'Open Sans'; font-weight:400; font-size:16px; margin:0 0 20px 0; border:none;}
-
   .texte{color:#5a6060; font-family:'Open Sans'; font-size:14px; margin:0 0 50px 0; line-height:24px; text-align: justify; }
+
   .texte{color:#5a6060; font-family:'Open Sans'; font-size:14px; margin:0 0 50px 0; line-height:24px; }
   .texteleft{color:#5a6060; font-family:'Open Sans'; font-size:14px; display:block; float:left; width:340px; margin:0 30px 50px 0;}
   .texteleft{color:#5a6060; font-family:'Open Sans'; font-size:14px; display:block; float:left; width:340px; margin:0 30px 50px 0;}
Line 70: Line 70:
<div class="maincontent" style="width: 720px; margin: 25px 0 25px 0; float: right;">
<div class="maincontent" style="width: 720px; margin: 25px 0 25px 0; float: right;">
-
  <h1 class="title1">Project</h1>
 
-
   <h2 class="title2">Overview</h2>
+
   <h1 class="title1">Overview</h1>
-
   <h3 class="title3">Why <i>E.calculus ?</i></h3>
+
   <h2 class="title2">Why <i>E.calculus ?</i></h2>
   <p class="texte">
   <p class="texte">
-
When we first made brainstorming trying to define our iGEM project for this year, we realized that addition is present everywhere in living world, as example in many intracellular regulations and controls, or even in control of metabolic pathways. So we decided to create a n-bit bacterial full adder, which could be adjustable by the user, able to transmit a carry if needed. Considering every iGEM projects already based on the adder concept, and using the all new systems of logic recombinatorial gates, we aim to create a robust and strong device able to be implemented on bacterial chassis. We also want to prove (demonstrate / reveal / show) in this project how recombinatorial gates could be considered as the easiest and strongest way to build genetic logic gates, which could be used together in the creation of many genetic devices.</p>
+
During our brainstorming meetings to decide what would be our iGEM project, many ideas on building artificial regulations came out. We realized that most biological regulation systems perform highly specific implicit operations (greater than, lower than, if, etc.). However, none of them are prone to execute real mathematical operations. We therefore decided to create a n-bit bacterial full adder which could be operated by the user and able to transmit a carry if needed. Considering that some of the previous iGEM projects tackled the bacterial adder concept, we envisioned a new strategy using recombination-based logic gates. The most important achievements were to demonstrate that recombination-based switches represent simple yet highly efficient genetic logic gates modules that can be further integrated into more complex genetic devices. Besides the full adder idea, these genetic devices should lead to substantial progresses in the design of artificially regulated synthetic pathways. </p>
-
   <h3 class="title3">From TI83+ to <i>E.calculus</i></h3>
+
   <h2 class="title2">From TI83+ to <i>E. calculus</i></h2>
-
   <p class="texte">An electronic full-adder consists of the association of 5 logic gates (2 XOR gates, 2 AND gates, and 1 OR gate) which are able to account for 3 input signals (2 input bits and the last stage carry) and give the right answer to the user. (link to “full adder” part)
+
   <p class="texte">An electronic full-adder consists of the association of 5 logic gates (2 XOR gates, 2 AND gates, and 1 OR gate) which are able to receive 3 input signals (2 input bits and the last stage carry) and give the right answer to the user. <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/full-adder">(View "Full Adder" Part)</a></li>
</p>
</p>
    
    
-
   <img src="https://static.igem.org/mediawiki/2013/b/b7/Insa-toulouse2013-phototestpage.jpg" class="imgcontent" />
+
   <img src="https://static.igem.org/mediawiki/2013/9/9b/400px-Full_Adder.png" class="imgcontent" />
-
 
+
-
  <p class="texteleft"><span class="spantitle">lorem ipsum</span></br>
+
-
<br>Mauris ac suscipit erat, sit amet blandit lectus. Fusce placerat, lectus at suscipit viverra, lorem lorem mattis turpis, id pharetra velit nunc vitae velit. Etiam ultrices aliquam ligula, sed ultrices nibh vulputate non. Ut dapibus arcu luctus, suscipit urna et, imperdiet magna. Curabitur tristique sed diam non elementum. Sed lectus urna, consequat quis porta eu, ultrices in nunc. Aenean vitae elit neque.<p>
+
-
 
+
-
  <p class="texteright"><span class="spantitle">lorem ipsum</span></br>
+
-
<br>Mauris ac suscipit erat, sit amet blandit lectus. Fusce placerat, lectus at suscipit viverra, lorem lorem mattis turpis, id pharetra velit nunc vitae velit. Etiam ultrices aliquam ligula, sed ultrices nibh vulputate non. Ut dapibus arcu luctus, suscipit urna et, imperdiet magna. Curabitur tristique sed diam non elementum. Sed lectus urna, consequat quis porta eu, ultrices in nunc. Aenean vitae elit neque.</p>
+
-
 
+
-
<div class="clear"></div>
+
 +
  <p class="texte">
 +
  With <i>E. calculus</i> we tried to link the various logic gate genetic devices to:
   <div class="list">
   <div class="list">
-
   
+
     <ul class="circlearrow">
-
     <ul class="arrow">
+
       <li>A semi-artificial input system converting 0 and 1 input signals (absence and presence of blue and red lights) into biological signals.<a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/input">(View "Input" Part)</a>
-
       <li>Quisque at massa ipsum</li>
+
       </li>
-
      <li>Maecenas a lorem augue, egestas </li>
+
-
      <li>Cras vitae felis at lacus ele </li>
+
-
      <li>Etiam auctor diam pellentesque </li>
+
-
       <li>Nulla ac massa at dolor </li>
+
-
    </ul>
+
-
 
+
   </div>
   </div>
-
 
   <div class="list">
   <div class="list">
-
   
 
     <ul class="circlearrow">
     <ul class="circlearrow">
-
       <li>Quisque at massa ipsum</li>
+
       <li>The production and diffusion of a bacterial signalling molecule, acyl homoserine lactone (AHL) for the carry, transmitting the information of from one cell to the other <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/carry">(View "Carry" Part)</a>
-
      <li>Maecenas a lorem augue, egestas </li>
+
       </li>
-
      <li>Cras vitae felis at lacus ele </li>
+
-
      <li>Etiam auctor diam pellentesque </li>
+
-
       <li>Nulla ac massa at dolor </li>
+
     </ul>
     </ul>
-
 
   </div>
   </div>
-
 
   <div class="list">
   <div class="list">
-
   
+
     <ul class="circlearrow">
-
     <ul class="arrow">
+
       <li>An easily readable result of the various operations consisting of a clearly visible output pigment: the Red Fluorescent Protein (RFP) <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/output">(View "Output" Part)</a>
-
       <li>Quisque at massa ipsum</li>
+
       </li>
-
      <li>Maecenas a lorem augue, egestas </li>
+
-
      <li>Cras vitae felis at lacus ele </li>
+
-
      <li>Etiam auctor diam pellentesque </li>
+
-
       <li>Nulla ac massa at dolor </li>
+
     </ul>
     </ul>
-
 
   </div>
   </div>
-
 
+
</p>
-
<div class="clear"></div>
+
-
 
+
-
  <h3 class="title3">TITRE 3 lorem ipsum</h3>
+
-
 
+
-
  <p class="texte">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut et dolor turpis. Suspendisse interdum, dolor eu  ultricies dignissim, arcu sapien placerat quam, ut bibendum lorem nibh id magna. Nunc condimentum lectus at diam tempus sodales. Nullam scelerisque auctor tellus, nec auctor ante elementum sit amet. Aenean venenatis velit eget porttitor laoreet. Aliquam aliquam libero in ante imperdiet vehicula. Donec nibh urna, tincidunt quis condimentum at, tempus sed sapien. Nunc elit dui, dignissim tincidunt mi non, ultrices ultricies erat.</p>
+
-
 
+
-
  <table class="tablecontent">
+
-
 
+
-
<tr style="background-color:#20a8da; height:50px; color:#ffffff;" >
+
-
<td style="border-bottom:4px solid #e5e6e6; border-top-left-radius:9px;">TR  Title</td>
+
-
<td style="border-bottom:4px solid #e5e6e6;">TR  Title</td>
+
-
<td style="border-bottom:4px solid #e5e6e6; border-top-right-radius:9px;">TR  Title</td>
+
-
</tr>
+
-
<tr>
+
-
<td style="border-right:1px solid #e5e6e6;">Quisque</td>
+
-
<td style="border-right:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
<td>Maecenas a lorem augue</td>
+
-
</tr>
+
-
<tr style="border-top:1px solid #e5e6e6">
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Quisque</td>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
<td style="border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
</tr>
+
-
<tr>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Quisque</td>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
<td style="border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
</tr>
+
-
<tr>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Quisque</td>
+
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
<td style="border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
-
</tr>
+
-
</table>
+
-
 
+

Latest revision as of 15:28, 4 October 2013

logo


Overview

Why E.calculus ?

During our brainstorming meetings to decide what would be our iGEM project, many ideas on building artificial regulations came out. We realized that most biological regulation systems perform highly specific implicit operations (greater than, lower than, if, etc.). However, none of them are prone to execute real mathematical operations. We therefore decided to create a n-bit bacterial full adder which could be operated by the user and able to transmit a carry if needed. Considering that some of the previous iGEM projects tackled the bacterial adder concept, we envisioned a new strategy using recombination-based logic gates. The most important achievements were to demonstrate that recombination-based switches represent simple yet highly efficient genetic logic gates modules that can be further integrated into more complex genetic devices. Besides the full adder idea, these genetic devices should lead to substantial progresses in the design of artificially regulated synthetic pathways.

From TI83+ to E. calculus

An electronic full-adder consists of the association of 5 logic gates (2 XOR gates, 2 AND gates, and 1 OR gate) which are able to receive 3 input signals (2 input bits and the last stage carry) and give the right answer to the user. (View "Full Adder" Part)

With E. calculus we tried to link the various logic gate genetic devices to:

  • A semi-artificial input system converting 0 and 1 input signals (absence and presence of blue and red lights) into biological signals.(View "Input" Part)
  • The production and diffusion of a bacterial signalling molecule, acyl homoserine lactone (AHL) for the carry, transmitting the information of from one cell to the other (View "Carry" Part)
  • An easily readable result of the various operations consisting of a clearly visible output pigment: the Red Fluorescent Protein (RFP) (View "Output" Part)