Team:Leeds
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==The Leeds 2013 iGEM team== | ==The Leeds 2013 iGEM team== | ||
- | [[File:Leeds_Map.png| | + | [[File:Leeds_Map.png|300px|right|The Kings in the North|link=http://maps.google.co.uk/maps?q=Leeds+map&oe=utf-8&client=firefox-a&ie=UTF-8&ei=VHDuUci7J7CT0AWYoYCQDg&ved=0CAoQ_AUoAg|frameless]] |
We are the Leeds 2013 iGEM team, We are a group of undergrads from various courses at the University of Leeds, all with a passion for synthetic biology! | We are the Leeds 2013 iGEM team, We are a group of undergrads from various courses at the University of Leeds, all with a passion for synthetic biology! | ||
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The University of Leeds is a [http://en.wikipedia.org/wiki/Red_brick_university British Red Brick] and member of the Russell Group and was officially formed as an independent university via Royal Charter from King Edward VII in 1904. | The University of Leeds is a [http://en.wikipedia.org/wiki/Red_brick_university British Red Brick] and member of the Russell Group and was officially formed as an independent university via Royal Charter from King Edward VII in 1904. | ||
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<div><html><video poster="https://static.igem.org/mediawiki/2013/f/f3/Leeds_SummaryVid.jpg" controls> | <div><html><video poster="https://static.igem.org/mediawiki/2013/f/f3/Leeds_SummaryVid.jpg" controls> | ||
<source src="https://static.igem.org/mediawiki/2013/b/b4/Leeds_SummaryVid.mp4" type='video/mp4'/> | <source src="https://static.igem.org/mediawiki/2013/b/b4/Leeds_SummaryVid.mp4" type='video/mp4'/> |
Revision as of 01:18, 5 October 2013
A biological system designed to detect the presence of pathogens in water samplesMicro-Beagle is a novel reporter system for E-coli that, as an iGEM first, has been designed to dynamically detect arbitrary target solids (including other cells) through a mechanism activated by cell surface binding. Micro-Beagle was initially developed to address the need for a low cost, energy efficient and robust tool to be used to assess the effectiveness of current water purification systems. As the Micro-Beagle is intended to operate at a low cost it is ideal for use in low-income areas where the testing of water purification techniques cannot be maintained at an acceptable frequency. Micro-Beagle is designed to also be a modular system, utilising Ice Nucleation Protein to express and position target-binding peptides on the cell surface. Target binding induces membrane stress that activates the Cpx signalling pathway, and Micro-Beagle thus utilises a promoter from this pathway (pCpxR) to initiate expression of a reporter protein, such as GFP. As a proof of concept, we have used silica beads as a model diagnostic target (a pathogen surrogate) and the silica-binding “Si4” sequence as the target-binding peptide. We foresee Micro-Beagle being adapted for both the detection of waterborne pathogens and a variety of other diagnostic applications, and we envision future multisensor Micro-Beagles in which diverse pathogens can be simultaneously and quantitatively measured from a single water sample.
The Leeds 2013 iGEM teamWe are the Leeds 2013 iGEM team, We are a group of undergrads from various courses at the University of Leeds, all with a passion for synthetic biology!
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