6/3

Digest:

B0015

pRhl

pLas

RBS-mCherry

RBS-RhlR1

RBS-RhlR2

RBS-LasR1

RBS-LasR2

RBS-GFPmut

RBS-mRFP

Ligation overnight:

RBS-RhlR1-tt

RBS-LasR1-tt

pRhl-RBS-RhlR2

pLas RBS-LasR2

RBS-GFP-tt

RBS-mRFP-tt

RBS-mCherry-tt

6/4

Transform:

RBS-RhlR1-tt

RBS-LasR1-tt

pRhl-RBS-RhlR2

pLas RBS-LasR2

RBS-GFP-tt

RBS-mRFP-tt

RBS-mCherry-tt

6/5

Inoculation & Incubation

RBS-RhlR1-tt

RBS-LasR1-tt

pRhl-RBS-RhlR2

pLas RBS-LasR2

RBS-GFP-tt

RBS-mRFP-tt

RBS-mCherry-tt

6/7

Check:

RBS-RhlR1-tt

RBS-LasR1-tt

pRhl-RBS-RhlR2

pLas RBS-LasR2

RBS-GFP-tt

RBS-mRFP-tt

RBS-mCherry-tt

6/7

Get Plasmid miniprep:

A3 : 195.1 ng / μL

A4 : 218.9 ng / μL

B4 : 196.1 ng / μL

B5 : 226.2 ng / μL

C1 : 152.0 ng / μL

C4 : 148.3 ng / μL

D3 :198.3 ng / μL

D5 : 209.2 ng / μL

E1 : 226.1 ng / μL

E5 : 160.4 ng / μL

F1 : 185.4 ng / μL

F2 : 191.7 ng / μL

G3: 197.8 ng / μL

G4: 216.0 ng / μL