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Team:NTU-Taida/Parts
From 2013.igem.org
Part
Contents |
Note
Some plasmids are not submitted due to similar parts in registry or time limit while they are still important fragments in our projects and we still lists them below.
Also, BBa_K575024 and BBa_K575033 submitted from the Northwestern help a lot.
Original Biobrick
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157000 | Regulatory | promoter sequence for PQS quorum sensing | 341 |
| BBa_K1157001 | Coding | PQSR | 999 |
| BBa_K1157003 | Coding | AbaR | 597 |
Reporter
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157005 | Reporter | Luciferase generator | 1094 |
| Reporter | RBS-mTagBFP-tt | 849 | |
| Reporter | RBS-mCherry-tt | 858 | |
| Reporter | RBS-GFP-tt | 867 |
Protein Generator
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157007 | Coding | Quorum sensing receptor pqsR generator | 1157 |
| BBa_K1157017 | Composite | pPQS-GFPmut3 | 1227 |
| BBa_K1157019 | Composite | Pc-PQSR | 1200 |
| Composite | RBS-RhlR-tt | 873 | |
| Composite | RBS-LasR-tt | 867 | |
| Composite | RBS-CinR-tt | 931 | |
| Composite | RBS-AbaR-tt | 741 |
Without positive feedback circuits
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157010 | Composite | Pc-RhlR-mCherry | 1871 |
| BBa_K1157016 | Composite | Pc-LasR-pLas-BFP | 1960 |
| BBa_K575033 | Composite | PRhl-GFP-Pc-RhlR | 1600 |
| BBa_K575024 | Composite | Plas-GFP-Pc-LasR | 1701 |
| BBa_K1157008 | Composite | pLas-BFP-Pc-LasR | 2018 |
| Composite | pPQS-GFP-Pc-PQSR | 2401 |
Positive Feedback circuit
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157020 | Composite | Pc-RhlR-pRhl-RhlR-GFPmut3 | 2635 |
| BBa_K1157011 | Composite | Pc-RhlR-pRhl-RhlR-BFP | 2620 |
| BBa_K1157012 | Composite | Pc-RhlR-pRhl-RhlR-luciferase | 2851 |
| BBa_K1157018 | Composite | pRhl-RhlR-mCherry-Pc-RhlR | 2629 |
| BBa_K1157021 | Composite | pRhl-RhlR-GFP-Pc-RhlR | 2635 |
| BBa_K1157014 | Composite | pLas-LasR-GFPmut3-Pc-LasR | 2727 |
Negative Regulation
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157013 | Composite | pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI | 3627 |
| BBa_K1157015 | Composite | pConst-Rhl-pRhl-RhlR-CI-pCI-mCherry | 3627 |
