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Team:NTU-Taida/Parts
From 2013.igem.org
Part
Contents |
Original Biobrick
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157000 | Regulatory | promoter sequence for PQS quorum sensing | 341 |
| BBa_K1157001 | Coding | PQSR | 999 |
| BBa_K1157003 | Coding | AbaR | 597 |
Reporter
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157005 | Reporter | Luciferase generator | 1094 |
| Reporter | RBS-mTagBFP-tt | 849 | |
| Reporter | RBS-mCherry-tt | 858 | |
| Reporter | RBS-GFP-tt | 867 | |
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Protein Generator
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157007 | Coding | Quorum sensing receptor pqsR generator | 1157 |
| BBa_K1157017 | Composite | pPQS-GFPmut3 | 1227 |
| BBa_K1157019 | Composite | Pc-PQSR | 1200 |
| RBS-RhlR-tt | 873 | ||
| RBS-LasR-tt | 867 | ||
| RBS-CinR-tt | 931 | ||
| RBS-AbaR-tt | 741 |
Without positive feedback circuits
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157010 | Composite | Pc-RhlR-mCherry | 1871 |
| BBa_K1157016 | Composite | Pc-LasR-pLas-BFP | 1960 |
| BBa_K575033 | Composite | PRhi-GFP-Pc-RhlR | 1600 |
| BBa K575024 | Composite | Plas-GFP-Pc-LasR | 1701 |
| BBa_K1157008 | Composite | pLas-BFP-Pc-LasR | 2018 |
| pPQS-GFP-Pc-PQSR | 2401 |
Positive Feedback circuit
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157020 | Composite | Pc-RhlR-pRhl-RhlR-GFPmut3 | 2635 |
| BBa_K1157011 | Composite | Pc-RhlR-pRhl-RhlR-BFP | 2620 |
| BBa_K1157012 | Composite | Pc-RhlR-pRhl-RhlR-luciferase | 2851 |
| BBa_K1157018 | Composite | pRhl-RhlR-mCherry-Pc-RhlR | 2629 |
| BBa_K1157021 | Composite | pRhl-RhlR-GFP-Pc-RhlR | 2635 |
| BBa_K1157014 | Composite | pLas-LasR-GFPmut3-Pc-LasR | 2727 |
Negative Regulation
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K1157013 | Composite | pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI | 3627 |
| BBa_K1157015 | Composite | pConst-Rhl-pRhl-RhlR-CI-pCI-mCherry | 3627 |
