Team:NTU-Taida/Parts

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Part



Contents

Original Biobrick

NameTypeDescriptionLength
BBa_K1157000Regulatorypromoter sequence for PQS quorum sensing341
BBa_K1157001CodingPQSR999
BBa_K1157003CodingAbaR597

Reporter

NameTypeDescriptionLength
BBa_K1157005ReporterLuciferase generator1094
 ReporterRBS-mTagBFP-tt849
    
 ReporterRBS-mCherry-tt858
    
 ReporterRBS-GFP-tt867
   

Protein Generator

NameTypeDescriptionLength
BBa_K1157007CodingQuorum sensing receptor pqsR generator1157
BBa_K1157017CompositepPQS-GFPmut31227
BBa_K1157019CompositePc-PQSR1200
  RBS-RhlR-tt873
    
  RBS-LasR-tt867
  RBS-CinR-tt931
  RBS-AbaR-tt741

Without positive feedback circuits

NameTypeDescriptionLength
BBa_K1157010CompositePc-RhlR-mCherry1871
BBa_K1157016CompositePc-LasR-pLas-BFP1960
BBa_K575033CompositePRhi-GFP-Pc-RhlR1600
BBa K575024CompositePlas-GFP-Pc-LasR1701
BBa_K1157008CompositepLas-BFP-Pc-LasR2018
  pPQS-GFP-Pc-PQSR2401

Positive Feedback circuit

NameTypeDescriptionLength
BBa_K1157020CompositePc-RhlR-pRhl-RhlR-GFPmut32635
BBa_K1157011CompositePc-RhlR-pRhl-RhlR-BFP2620
BBa_K1157012CompositePc-RhlR-pRhl-RhlR-luciferase2851
BBa_K1157018CompositepRhl-RhlR-mCherry-Pc-RhlR2629
BBa_K1157021CompositepRhl-RhlR-GFP-Pc-RhlR2635
BBa_K1157014CompositepLas-LasR-GFPmut3-Pc-LasR2727

Negative Regulation

NameTypeDescriptionLength
BBa_K1157013CompositepCI-mCherry-pConst-RhlR-pRhl-RhlR-CI3627
BBa_K1157015CompositepConst-Rhl-pRhl-RhlR-CI-pCI-mCherry3627