Team:Paris Saclay/Notebook/July/10

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Notebook : July 10

Summary:

For regulation system:

  • prepared the solution of BioBrick fnr repressor in PsB1C3 plasmid for DNA sequencing.
  • the terminator transformation of yesterday did not work, a second transformation had been done for it.
  • The transformation for RBS+LacZ+terminator plasmid into competent cells was performed.
  • after the transformation PSB3K3 plasmid in competent cells, these cells were cultured in a liquid nutritive medium.


For PSBs sensor system:

  • the ligation products were transformed into competent cells and were cultured on solid

medium with their specific antibiotics.

Lab work

constructing



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