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Team:TU-Delft/All
From 2013.igem.org
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| + | <div style="margin-left:30px;margin-right:30px; width:900px;float:left;"> | ||
| + | <h2 align="center">Planning</h2> | ||
| + | <html> | ||
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| + | <p align="justify"> | ||
| + | Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. Both the detailed cloning strategies related to each module and the final constructs can be viewed in the following links: | ||
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| + | <ul style="list-style-type: circle"> | ||
| + | <li><a href="https://2013.igem.org/Team:TU-Delft/PeptideProduction" style="text-decoration: none"><font color="#0080FF" size="3">Peptide Production Cloning Schemes</font></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:TU-Delft/KillSwitchPlanning" style="text-decoration: none"><font color="#0080FF" size="3">Kill Switch Cloning Schemes</font></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:TU-Delft/Sensing Device" style="text-decoration: none"><font color="#0080FF" size="3">Sensing Device Cloning Schemes</font></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:TU-Delft/FinalConstructs" style="text-decoration: none"><font color="#0080FF" size="3">Final Constructs</font></a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:TU-Delft/All" style="text-decoration: none"><font color="#0080FF" size="3">All Cloning Schemes</font></a> </li> | ||
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| + | </ul> | ||
| + | </p> | ||
| + | |||
| + | </html> | ||
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position: relative; | position: relative; | ||
/*Instead of height we will use padding*/ | /*Instead of height we will use padding*/ | ||
| - | padding-bottom: -1121px; /*That helps bring the labels down*/ | + | /*padding-bottom: -1121px;*/ /*That helps bring the labels down*/ |
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.slider>img{ | .slider>img{ | ||
position: absolute; | position: absolute; | ||
| - | left: | + | left: 5px; top: 5px; |
transition: all 0.5s; | transition: all 0.5s; | ||
} | } | ||
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.slider label { | .slider label { | ||
/*Lets add some spacing for the thumbnails*/ | /*Lets add some spacing for the thumbnails*/ | ||
| - | margin: 10px 0 0 | + | margin: 10px 0 0 815px; |
border: 3px solid #999; | border: 3px solid #999; | ||
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<input type="radio" name="slide_switch" id="id1" checked="checked"/> | <input type="radio" name="slide_switch" id="id1" checked="checked"/> | ||
<label for="id1"> | <label for="id1"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/e/e3/Page1Cloning.jpg |
| + | " width="50px" height="63px" /> | ||
</label> | </label> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/e/e3/Page1Cloning.jpg |
| + | " width="700px"/> | ||
<!--Lets show the second image by default on page load--> | <!--Lets show the second image by default on page load--> | ||
<input type="radio" name="slide_switch" id="id2" /> | <input type="radio" name="slide_switch" id="id2" /> | ||
<label for="id2"> | <label for="id2"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/9/90/Page2Cloning.jpg |
| + | "width="50px" height="63px" /> | ||
</label> | </label> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/9/ | + | <img src="https://static.igem.org/mediawiki/2013/9/90/Page2Cloning.jpg |
| + | " width="700px" /> | ||
<input type="radio" name="slide_switch" id="id3" /> | <input type="radio" name="slide_switch" id="id3" /> | ||
<label for="id3"> | <label for="id3"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/1/1d/Page3Cloning.jpg |
| + | " width="50px" height="63px" /> | ||
</label> | </label> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/1/1d/Page3Cloning.jpg |
| + | "width="700px" /> | ||
<input type="radio" name="slide_switch" id="id4" /> | <input type="radio" name="slide_switch" id="id4" /> | ||
<label for="id4"> | <label for="id4"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/5/52/Page4Cloning.jpg |
| + | " width="50px" height="63px" /> | ||
</label> | </label> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/5/52/Page4Cloning.jpg |
| + | " width="700px" /> | ||
<input type="radio" name="slide_switch" id="id5" /> | <input type="radio" name="slide_switch" id="id5" /> | ||
<label for="id5"> | <label for="id5"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/5/55/Page5Cloning.jpg |
| + | " width="50px" height="63px" /> | ||
</label> | </label> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/5/55/Page5Cloning.jpg |
| + | " width="700px" /> | ||
<input type="radio" name="slide_switch" id="id6" /> | <input type="radio" name="slide_switch" id="id6" /> | ||
<label for="id6"> | <label for="id6"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/0/0b/Page6Cloning.jpg |
| + | " width="50px" height="63px" /> | ||
</label> | </label> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/0/0b/Page6Cloning.jpg |
| + | |||
| + | " width="700px" /> | ||
<input type="radio" name="slide_switch" id="id7" /> | <input type="radio" name="slide_switch" id="id7" /> | ||
<label for="id7"> | <label for="id7"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/e/e5/Page7Cloning.jpg |
| + | " width="50px" height="63px" /> | ||
</label> | </label> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/e/e5/Page7Cloning.jpg |
| + | " width="700px" /> | ||
<input type="radio" name="slide_switch" id="id8" /> | <input type="radio" name="slide_switch" id="id8" /> | ||
<label for="id8"> | <label for="id8"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/1/19/Page8Cloning.jpg |
| + | " width="50px" height="63px" /> | ||
</label> | </label> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/1/19/Page8Cloning.jpg |
| + | " width="700px" /> | ||
<input type="radio" name="slide_switch" id="id9" /> | <input type="radio" name="slide_switch" id="id9" /> | ||
<label for="id9"> | <label for="id9"> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/7/ | + | <img src="https://static.igem.org/mediawiki/2013/7/75/Page9Cloning.jpg |
| + | " width="50px" height="63px" /> | ||
| + | </label> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/7/75/Page9Cloning.jpg | ||
| + | " width="700px" /> | ||
| + | <input type="radio" name="slide_switch" id="id10" /> | ||
| + | <label for="id10"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/e/e7/Page10Cloning.jpg" width="50px" height="63px" /> | ||
</label> | </label> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/e/e7/Page10Cloning.jpg" width="700px" /> |
</div> | </div> | ||
<script src="http://thecodeplayer.com/uploads/js/prefixfree.js" type="text/javascript"></script> | <script src="http://thecodeplayer.com/uploads/js/prefixfree.js" type="text/javascript"></script> | ||
| + | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
| + | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
| + | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
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</html> | </html> | ||
Latest revision as of 10:38, 29 September 2013
Planning
Project 'Peptidor' deals with various cloning schemes into E.coli cells. They are made to sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and then start producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Several bio-bricks from the parts registry were used to clone the E. coli. There are many test constructs made in order to test and characterize each module. Both the detailed cloning strategies related to each module and the final constructs can be viewed in the following links:
- Peptide Production Cloning Schemes
- Kill Switch Cloning Schemes
- Sensing Device Cloning Schemes
- Final Constructs
- All Cloning Schemes
