Team:TU-Delft/Planning

From 2013.igem.org

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Our project deals with <i>E.coli</i> cells which sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and starts producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>.  
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Project 'Peptidor' deals with various cloning schemes into <i>E.coli</i> cells. They are made to sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and then start producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Several bio-bricks from the parts registry were used to clone the <i>E. coli</i>. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy is as follows:
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<img src="https://static.igem.org/mediawiki/2013/e/e4/Under-Construction.jpg" height=300px>
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<br> <h4>AIP test Construct </h4> <br>
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<img src="https://static.igem.org/mediawiki/2013/d/d4/AIP-test-constructs.jpg" height=600px>
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Revision as of 22:01, 15 September 2013


Planning

Project 'Peptidor' deals with various cloning schemes into E.coli cells. They are made to sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and then start producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Several bio-bricks from the parts registry were used to clone the E. coli. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy is as follows:


AIP test Construct