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Team:TU-Delft/Planning
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Planning
Project 'Peptidor' deals with various cloning schemes into E.coli cells. They are made to sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and then start producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Several bio-bricks from the parts registry were used to clone the E. coli. There are many test constructs made in order to test and characterize each module. A detailed cloning strategy is as follows:
His 6 - SUMO Peptide construct
Peptide Production
AIP test Construct
