Team:TU-Munich/HumanPractice/Media

From 2013.igem.org

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==Science Communication & Publicity==
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"A scientist has to be neutral in his search for the truth, but he cannot be neutral as to the use of that truth when found. If you know more than other people, you have more responsibility, rather than less."
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<div class="author">- Baron C.P. Snow</div>
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The importance of science communication cannot be stressed enough. As students, we spend huge amounts of time at the TUM life science campus and at the lab, allowing us to dig deep into our subjects, focus on our studies and enjoy the privilege of an environment designed to support us in the pursuit of our scientific interests.
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But our knowledge and experiences wouldn't be of much use if we didn't communicate and share them. New technology can't find the support it needs if no one apart from a few experts understands what it is all about and many great ideas wouldn't have seen the light of day without the creative synergies that develop through interdisciplinary dialog. Communicating ideas to people with different backgrounds not only provides valuable new input and views from way outside the box: The necessity to take a step back and explain the bigger picture also brings us to look at our work and intentions from a whole new perspective, deepens our understanding and brings up aspects previously missed.
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Furthermore, a well informed public creates the kind of open minded environment that catalyzes bold thinking and allows great ideas to thrive. As undergrads, we're still halfway between the experts of our fields and the public. So as part of our project, we set out to mediate between those two worlds, to inspire and get inspired.
==Deutsches Museum Munich==
==Deutsches Museum Munich==
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[[File:TUM13 German_Museum.jpg|thumb|right|250px| Figure 1: Our poster for the museum (german)]]
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[[File:TUM13 German_Museum.jpg|thumb|right|250px| '''Figure 1:''' Our poster for the museum (german)]]
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We had the chance to introduce the Physco Filter and our iGEM team to the public at the visitors lab at the German Museum of Munich. Green biotechnology is perceived rather negatively in Germany, but the majority of expressed concers relates to applications in agriculture and food. So in addition to presenting our project and answering a lot of interested questions about sythetic biology, we asked people about their views to see if projects like the Physco Filter can bring a different perspective into the public debate about biotechnology.
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We had the chance to introduce the PhyscoFilter and our iGEM team to the public at the visitors lab at the German Museum of Munich. Green biotechnology is perceived rather negatively in Germany, but the majority of expressed concerns relates to applications in agriculture and food. So additional to presenting our project and answering a lot of interested questions about synthetic biology, we asked people about their views to see if projects like the PhyscoFilter can bring a different perspective into the public debate about biotechnology.
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Because of the Octoberfest, we had a large and very international audience with whom we had very interesting discussions. We had prepared a poster aimed at a larger audience in German language, but we could have needed posters in English, Italian, French, Russian, Chinese and Hebrew as well and had the chance to use all the forgein language skills we had. The feedback we received was much more positive than expected. People complimented our idea and considered this application of green biotechnology as much more feasible due to its thorough biosafety profile.
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Because of the Oktoberfest, we had a large and very international audience with whom we had very interesting discussions. We had prepared a poster aimed at a larger audience in German language, but we could have needed posters in English, Italian, French, Russian, Chinese and Hebrew as well and had the chance to use all the foreign language skills we had. The feedback we received was much more positive than expected. People appreciated our idea and considered this application of green biotechnology as much more feasible due to its thorough biosafety profile.
We really enjoyed this campaign because of all the valuable feedback and the large diversity of input we got. It was very inspiring to meet and talk to all those people and once again, our iGEM activities enriched our views.
We really enjoyed this campaign because of all the valuable feedback and the large diversity of input we got. It was very inspiring to meet and talk to all those people and once again, our iGEM activities enriched our views.
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====PhyscoFilter presentation at the German Museum====
====PhyscoFilter presentation at the German Museum====
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==Survey on the public perception of biotechnology==
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==Survey on the public perception of SynBio==
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The public perception of biotechnology in Germany is traditionally not very positive. But how much do people know about sythetic biology and are red, white and green biotechnology perceived equally postive or negative? On iGEM day Germany, we set out to inform, explain and investigate. How much impact does the pollution of water through pharmaceuticals and hormones have on people´s private life as well as globally according to public opinion? And would projects like our Physco Filter that adresses these problems increase the acceptance of sythetic biology or not? Would people want to use our filter or support its use in public sewage plants?
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The public perception of biotechnology in Germany is traditionally not very positive. But how much do people know about synthetic biology? Are red, white and green biotechnology perceived equally positive or negative? On the iGEM-day-Germany, we set out to inform, explain and investigate. How much impact does the pollution of water through pharmaceuticals and hormones have on people's private life as well as globally according to public opinion? Are the projects addressing these problems able to increase the acceptance of synthetic biology? And would people want to use our filter or support its use in public sewage plants?
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[[File:Fragebogen_iGEM_Germany_day_Translated_copy_01.png|thumb|left|400px| Figure 2: Our questionnaire page 1 (translated to English)]] [[File:Fragebogen_iGEM_Germany_day_Translated_copy_02.png|thumb|right|400px| Figure 3: Our questionnaire page 2 (translated to English)]]
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[[File:Fragebogen_iGEM_Germany_day_Translated_copy_01.png|thumb|left|430px| '''Figure 2:''' Our questionnaire page 1 (translated to English)]] [[File:Fragebogen_iGEM_Germany_day_Translated_copy_02.png|thumb|right|430px| '''Figure 3:''' Our questionnaire page 2 (translated to English)]]  
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====Images from our information booth in the city of Freising====
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===Results:===
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[[File:Auswertung_Umfrage_translated_01.png|thumb|left|430px| '''Figure 4:''' Results page 1 (translated to English)]][[File:Auswertung_Umfrage_translated_02.png|thumb|right|430px| '''Figure 5:''' Results page 2 (translated to English)]]
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Green biotechnology is seen as far more controversial than industrial biotechnology, while medical applications are widely accepted. Most people feel personally concerned by water pollution and also perceive this problem as highly relevant in a global context. If synthetic biology can offer solutions for problems like these, people would be more likely to approve genetic engineering. Nearly two thirds would use a moss filter in their homes and 84% would agree with its use e.g. for hospital wastewater. We were happy to learn from the evaluation that our project is widely perceived as positive and that people found our survey and the conversations helpful to learn more about synthetic biology.
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====Our survey booth in the city of Freising====
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==13th Munich Science Days==
==13th Munich Science Days==
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http://www.muenchner-wissenschaftstage.de/2013/front_content.php
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November 16th to 19th we will be presenting our project at the [http://www.muenchner-wissenschaftstage.de/2013/front_content.php 13th Munich Science Days]. This popular public science fair offers workshops for school classes, public discussions, about 30 presentations and four theme nights, next to a large exhibition, tours and programs for kids.
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==Media coverage on iGEM 2013 project==
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==Speech about iGEM at the GoBiochem Team TU Munich==
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[[File:TUM13 Pressrelease.png|thumb|center|420px| Figure 3:]]
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[http://www.wzw.tum.de/index.php?id=185&no_cache=1&tx_ttnews%5Btt_news%5D=573 TUM WZW News] (german)
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[[File:TUM13 GoBiochem.jpg|thumb|right|250px| '''Figure 6:''' Speech at the GoBiochem Team TU Munich]]
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[[File:TUM13 Press_trinational.png|thumb|center|420px| Figure 3:]]
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* [http://www.tip-itp.eu/2013/09/igem-team-tu-munich-visiting-labs-of-the-tip-in-freiburg/ TIP News]
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To inform scientifically interested pupils about the iGEM competition, Leonie and Johanna gave a lecture on our project to students participating in the [http://www.ch.tum.de/gobiochem/ GoBiochem] program. This program is an initiative from several biochemistry and molecular biotechnology students of the TU Munich who give pupils, aged between 15 and 18, the opportunity to get an insight into the biochemistry degree both into theoretical and practical aspects. They listen to talks about biological and chemical topics, perform a  two-week introductive laboratory course and visit different TUM institutes to get an insight into real science.  
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[[File:TUM13_Biotechnologie_de.png|thumb|center|420px| Figure 3:]]
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Therefore the presentation about synthetic biology and the iGEM competition in particular was given to an ideal target audience to inspire a new generation.
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[http://www.biotechnologie.de/BIO/Navigation/DE/root,did=166602.html?listBlId=74462&] (german)
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==Media coverage on iGEM 2013 project==
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    <li><img src="https://static.igem.org/mediawiki/2013/a/a7/TUM13_Pressrelease.png" alt="<b>Figure 7:</b> Article in the <a href='http://www.wzw.tum.de/index.php?id=185&no_cache=1&tx_ttnews%5Btt_news%5D=573'>TUM WZW News</a>"/></li>
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    <li><img src="https://static.igem.org/mediawiki/2013/c/c6/TUM13_Biotechnologie_de.png" alt="<b>Figure 9:</b> Article in <a href='http://www.biotechnologie.de/BIO/Navigation/DE/root,did=166602.html?listBlId=74462&'>www.biotechnologie.de</a>"/></li>
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    <li><img src="https://static.igem.org/mediawiki/2013/c/cb/TUM13_Innovations-report.png" alt="<b>Figure 10:</b> Article on <a href='http://www.innovations-report.de/html/berichte/biowissenschaften_chemie/moos_sauberes_wasser_abwasserreinigung_physcofilter_220378.html'>www.innovations-report.de</a>"/></li>
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    <li><img src="https://static.igem.org/mediawiki/2013/8/82/TUM13_Schmude.JPG" alt="<b>Figure 11:</b> At the interview with Magdalena Schmude from <a href='http://www.dradio.de/dlf/'> Deutschlandfunk radio station</a>"/></li>
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    <li><img src="https://static.igem.org/mediawiki/2013/e/eb/TUM13_Deutschlandfunk.png" alt="<b>Figure 12:</b> Report from <a href='http://www.dradio.de/dlf/sendungen/forschak/2283279/'>Deutschlandfunk</a>"/></li>
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"A gateway to a lot of Synthetic Biology" - The part about our last years iGEM project is from minute 14.20 on. An interesting video from the beginning to the end by the way.
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[http://www.innovations-report.de/html/berichte/biowissenschaften_chemie/moos_sauberes_wasser_abwasserreinigung_physcofilter_220378.html] (german)
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== Introducing Synthetic Biology Kits at School ==
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[[File:TUM13_School_puote.png|center|900px]]
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===Introduction===
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[[File:TUM13_Schulkits.jpg|thumb|right|400px|Prepared School-Kits, ready for delivery!]]
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It is the first time for a TU Munich iGEM team to prepare „School-Kits“, to make it possible for disciples to take the first steps in Synthetic  Biology. The idea is to send a kit with all required reagents and lab-protocols, based on the experiments we designed, to educational institutions which are interested in inspiring young prospective people who are planning a career in science. Our experiments are designed in an easy way, so every disciple, independent of training level, is able to understand and take notice of the resulting phenomena which Synthetic Biology makes possible.
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===The School-kit===
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The reagents, which are necessary for the realization of the experiments are all found in the kit, except for competent cells, which have to be stored at -20°C. The kit includes the following reagents:
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{|cellspacing="0" border="1" right
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! align="center";| Reagent
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! align="center";| Is it existent in the kit?
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|LB-Medium
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|Reagents for about 3 L are in the kit
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|Yes
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|100 µl (10 ng/µl)
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|Yes
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|100 µl (10 ng/µl)
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|Yes
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|"Luciferase"-Plasmid
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|100 µl (10 ng/µl)
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|10 ml
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|2
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|25 ml
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As you may have noticed, we provide three different plasmids, which are used seperately in each designed experiment. The aim of the experiments is to perform the whole way from transformation, over cultivating cells, inducing the production of proteins and in the end demonstrate the conversion of a substrate by the produced enzyme.
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===Experiment Number 1===
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[[File:TUM13_RFP_Generator.jpg|thumb|right|400px|Results of Experiment Number 1]]
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The aim of the first experiment is to transform the "RFP-Generator"-Plasmid into ''Escherichia coli'' containing genes coding for Chloramphenicol resistance and a red fluorophor and to plate them on Chloramphenicol-Agarplates. The Agarplates, provided with the antibiotica Chloramphenicol, make a selection process possible, whose aim is to kill all bacteria which have not incorporated the "RFP-Generator"-Plasmid. The expression rate of the red fluorophor is controlled by a constitutive promotor, so the Red fluorescent Protein is expressed continuously. After just about one day of incubation time, the disciples are able to notice a change in colour of the bacteria from ocher to red.
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===Experiment Number 2===
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[[File:TUM13_Nanoluciferase.jpg|thumb|right|300px|Structure of the Nanoluciferase]]
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In the second experiment disciples should perform the transformation, learned in experiment one, with the "Luciferase"-Plasmid which encodes genes for Kanamycin resistance and a protein-fluorophor called Nanoluciferase. Another very important point is that the Nanoluciferasegene is subordinated to a L-Arabinose Operon. After the transformation of the DNA-Plasmid has taken place, discicples should learn how to cultivate bacteria in liquid LB-Medium and how to induce protein expression by using L-Arabinose as an inductor of the L-Arabinose promotor. The glow of the nanoluciferase can be observed after just a couple of hours.
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===Experiment Number 3===
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[[File:SchmolkerMollath.png|thumb|right|400px|Results of Experiment three]]
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In the third experiment, all aspects learned in the previous experiments come together. They are necessary to understand the following approaches. The "Banana-Odor"-Plasmid encodes an enzyme called Alcohol-Acetyltransferase I. This enzyme is able to convers Isoamylalcohol into Isoamylacetat, which spreads the odor of banana. Additionally there is found a resistance gene for Kanamycin and an inducible L-Arabinose promotor, which controls the expressions rate of the Alcohol-Acetyltransferase I. The disciples should transform the "Banana-Odor"-Plasmid into ''Escherichia coli'', cultivate the successfully transformed bacteria in a bigger scale and in the end, induce the protein expression using L-Arabinose. In the last step, the disciples should add the substrate Isoamylalcohol to the cell culture, so the produced enzyme can transform it into Isoamylacetat.
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===Target Audience===
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Our target audience have been secondary schools, which focus a biotechnical educational pathway, so all students have access to the required laboratory equipment for the experiments. Before sending the kits to the schools, we tested them in detail - with success. All schools we contacted, to spread our Synthetic Biology kits, are very enthusiastic concerning our idea of supporting young prospective scientists, so they are very excited to receive and use our kit!
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===Availability of the School-kit===
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We appreciate other interested schools  in trying our “School kits”. Do not be ashamed of contacting us, so we can supply you with our forward-looking Synthetic Biology “School-kit”!
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==Media coverage on iGEM 2012 project after the Jamboree==
==Media coverage on iGEM 2012 project after the Jamboree==
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[[File:TUM13 Beer_advocat.jpg|thumb|right|250px| Figure 3:]]
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[[File:TUM13 Beer_advocat.jpg|thumb|right|200px| '''Figure 13:''' "Munich Scientists Tinker with Yeast"]]
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An [http://aleszu.com/2013/01/munich-scientists-tinker-with-yeast article] in the [http://beeradvocate.com/community/threads/beeradvocate-magazine-72-pierogies-baileys-range-lawsons-vivant-buenos-aires-class-of-2012.59179/ BeerAdvocate magazine #72] written by Aleszu Bajak.
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This [http://aleszu.com/2013/01/munich-scientists-tinker-with-yeast/ article] by Aleszu Bajak covered our 2012 project in [http://beeradvocate.com/community/threads/beeradvocate-magazine-72-pierogies-baileys-range-lawsons-vivant-buenos-aires-class-of-2012.59179/ BeerAdvocate magazine, #72]
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<html><iframe width="430" height="270" style="border:1px #FFFFFF solide;" name="myiframe" scrolling="auto" frameborder="1" align=aus marginheight="5px" marginwidth="5px" src="http://www.youtube.com/embed/iRO0-fMIW9I?feature=player_detailpage" frameborder="0" allowfullscreen>
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"A gateway to a lot of Synthetic Biology" - The part about our last years iGEM project is from minute 14.20 on. An interesting video from the beginning to the end by the way.
"A gateway to a lot of Synthetic Biology" - The part about our last years iGEM project is from minute 14.20 on. An interesting video from the beginning to the end by the way.
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==References==
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==Representation on Social Media Networks==
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To be easily aproachable for people´s questions about iGEM, our project and our team and to stay in touch, we used our accounts on [https://www.facebook.com/pages/iGEM-TU-M%C3%BCnchen/149746078408629 Facebook] and [https://twitter.com/iGEM_TUM Twitter].
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[[http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984]]
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#[[http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984]] Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. ''Cell'', 37(2):629–33.
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<div style="width:450px;font-size:8px;text-align:right;float:right;"><iframe width="450" height="505" src="http://twitterforweb.com/iframe/twitterbox/iGEM_TUM.html?s=1,0,3,450,505,f4f4f4,0,c4c4c4,101010,1,1,336699" frameborder="0" scrolling="no" allowtransparency="1"></iframe>Created by: <a href="http://twitterforweb.com" target="_blank">twitter website widget</a></div>
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Latest revision as of 02:51, 29 October 2013


Science Communication & Publicity

"A scientist has to be neutral in his search for the truth, but he cannot be neutral as to the use of that truth when found. If you know more than other people, you have more responsibility, rather than less."

- Baron C.P. Snow

The importance of science communication cannot be stressed enough. As students, we spend huge amounts of time at the TUM life science campus and at the lab, allowing us to dig deep into our subjects, focus on our studies and enjoy the privilege of an environment designed to support us in the pursuit of our scientific interests.

But our knowledge and experiences wouldn't be of much use if we didn't communicate and share them. New technology can't find the support it needs if no one apart from a few experts understands what it is all about and many great ideas wouldn't have seen the light of day without the creative synergies that develop through interdisciplinary dialog. Communicating ideas to people with different backgrounds not only provides valuable new input and views from way outside the box: The necessity to take a step back and explain the bigger picture also brings us to look at our work and intentions from a whole new perspective, deepens our understanding and brings up aspects previously missed.

Furthermore, a well informed public creates the kind of open minded environment that catalyzes bold thinking and allows great ideas to thrive. As undergrads, we're still halfway between the experts of our fields and the public. So as part of our project, we set out to mediate between those two worlds, to inspire and get inspired.

Deutsches Museum Munich

Figure 1: Our poster for the museum (german)

We had the chance to introduce the PhyscoFilter and our iGEM team to the public at the visitors lab at the German Museum of Munich. Green biotechnology is perceived rather negatively in Germany, but the majority of expressed concerns relates to applications in agriculture and food. So additional to presenting our project and answering a lot of interested questions about synthetic biology, we asked people about their views to see if projects like the PhyscoFilter can bring a different perspective into the public debate about biotechnology.

Because of the Oktoberfest, we had a large and very international audience with whom we had very interesting discussions. We had prepared a poster aimed at a larger audience in German language, but we could have needed posters in English, Italian, French, Russian, Chinese and Hebrew as well and had the chance to use all the foreign language skills we had. The feedback we received was much more positive than expected. People appreciated our idea and considered this application of green biotechnology as much more feasible due to its thorough biosafety profile.

We really enjoyed this campaign because of all the valuable feedback and the large diversity of input we got. It was very inspiring to meet and talk to all those people and once again, our iGEM activities enriched our views.

PhyscoFilter presentation at the German Museum

Survey on the public perception of SynBio

The public perception of biotechnology in Germany is traditionally not very positive. But how much do people know about synthetic biology? Are red, white and green biotechnology perceived equally positive or negative? On the iGEM-day-Germany, we set out to inform, explain and investigate. How much impact does the pollution of water through pharmaceuticals and hormones have on people's private life as well as globally according to public opinion? Are the projects addressing these problems able to increase the acceptance of synthetic biology? And would people want to use our filter or support its use in public sewage plants?

Figure 2: Our questionnaire page 1 (translated to English)
Figure 3: Our questionnaire page 2 (translated to English)

Results:

Figure 4: Results page 1 (translated to English)
Figure 5: Results page 2 (translated to English)

Green biotechnology is seen as far more controversial than industrial biotechnology, while medical applications are widely accepted. Most people feel personally concerned by water pollution and also perceive this problem as highly relevant in a global context. If synthetic biology can offer solutions for problems like these, people would be more likely to approve genetic engineering. Nearly two thirds would use a moss filter in their homes and 84% would agree with its use e.g. for hospital wastewater. We were happy to learn from the evaluation that our project is widely perceived as positive and that people found our survey and the conversations helpful to learn more about synthetic biology.

Our survey booth in the city of Freising

13th Munich Science Days

November 16th to 19th we will be presenting our project at the [http://www.muenchner-wissenschaftstage.de/2013/front_content.php 13th Munich Science Days]. This popular public science fair offers workshops for school classes, public discussions, about 30 presentations and four theme nights, next to a large exhibition, tours and programs for kids.

Speech about iGEM at the GoBiochem Team TU Munich

Figure 6: Speech at the GoBiochem Team TU Munich

To inform scientifically interested pupils about the iGEM competition, Leonie and Johanna gave a lecture on our project to students participating in the [http://www.ch.tum.de/gobiochem/ GoBiochem] program. This program is an initiative from several biochemistry and molecular biotechnology students of the TU Munich who give pupils, aged between 15 and 18, the opportunity to get an insight into the biochemistry degree both into theoretical and practical aspects. They listen to talks about biological and chemical topics, perform a two-week introductive laboratory course and visit different TUM institutes to get an insight into real science. Therefore the presentation about synthetic biology and the iGEM competition in particular was given to an ideal target audience to inspire a new generation.

Media coverage on iGEM 2013 project

  • <b>Figure 7:</b> Article in the <a href='http://www.wzw.tum.de/index.php?id=185&no_cache=1&tx_ttnews%5Btt_news%5D=573'>TUM WZW News</a>
  • <b>Figure 8:</b> Article in <a href='http://www.tip-itp.eu/2013/09/igem-team-tu-munich-visiting-labs-of-the-tip-in-freiburg/'>Trinational Institute for Plant research News</a>/>
  • <b>Figure 9:</b> Article in <a href='http://www.biotechnologie.de/BIO/Navigation/DE/root,did=166602.html?listBlId=74462&'>www.biotechnologie.de</a>
  • <b>Figure 10:</b> Article on <a href='http://www.innovations-report.de/html/berichte/biowissenschaften_chemie/moos_sauberes_wasser_abwasserreinigung_physcofilter_220378.html'>www.innovations-report.de</a>
  • <b>Figure 11:</b> At the interview with Magdalena Schmude from <a href='http://www.dradio.de/dlf/'> Deutschlandfunk radio station</a>
  • <b>Figure 12:</b> Report from <a href='http://www.dradio.de/dlf/sendungen/forschak/2283279/'>Deutschlandfunk</a>

Media coverage on iGEM 2012 project after the Jamboree

Figure 13: "Munich Scientists Tinker with Yeast"

This [http://aleszu.com/2013/01/munich-scientists-tinker-with-yeast/ article] by Aleszu Bajak covered our 2012 project in [http://beeradvocate.com/community/threads/beeradvocate-magazine-72-pierogies-baileys-range-lawsons-vivant-buenos-aires-class-of-2012.59179/ BeerAdvocate magazine, #72]

"A gateway to a lot of Synthetic Biology" - The part about our last years iGEM project is from minute 14.20 on. An interesting video from the beginning to the end by the way.

Representation on Social Media Networks

To be easily aproachable for people´s questions about iGEM, our project and our team and to stay in touch, we used our accounts on Facebook and Twitter.