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Team:TU-Munich/Notebook/Materials
From 2013.igem.org
Cell lines
- E. coli XL-1 Blue (for molecular cloning)
- E. coli BL21 (for cytoplasmatic protein production
- P. patens clone 11 obtained from Prof. Reski (Freiburg University)
Reagents
Chemicals
| Reagent | Supplier | Location |
|---|---|---|
| 2-Mercaptoethanol | AppliChem GmbH | Darmstadt, Germany |
Kits and Standards
Plastic Disposables
Oligonucleotides
| Name | Sequence |
| EreA_for | 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘ |
| EreA-rev | 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘ |
| EreA_SDMI_for | 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘ |
| EreA_SDMI_rev | 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3' |
| EreA_SDMII_for | 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3' |
| EreA_SDMII_rev | 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3' |
| EreA_SDMIII_for | 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3' |
| EreA_SDMIII_rev | 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3' |
| EreB_for | 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3' |
| EreB-rev | 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3' |
| EreB_SDMI_for | 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3' |
| EreB_SDMI_rev | 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3' |
| EreB_SDMII_for | 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3' |
| EreB_SDMII_rev | 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3' |
| EreB_SDMIII_for | 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3' |
| EreB_SDMIII_rev | 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3' |
| TEV_fw | 5'- GCGCGTCTAGATGGCCGGCGGAGAAAGCTTGTTTAAGGGGC - 3' |
| TEV_rv | 5'- ATATAACCGGTATTCATGAGTTGAGTCGCTTCC - 3' |
| TEV_t387c_fw | 5'- ATGGTGTCAGACACTAGCTGCACATTCCCTTCATC - 3' |
| TEV_t387c_rv | 5'- GATGAAGGGAATGTGCAGCTAGTGTCTGACACCAT - 3' |
| N_split_TEV_fw | 5'- ATTCGCGGCCGCTTCTAG - 3' |
| N_split_TEV_rv | 5'- TTCGAAACCGGTAGTTTGGAAGTTGGTTGTCAC - 3' |
| C_split_TEV_fw | 5'- AAGCTTTCTAGATGGCCGGCAAGAGCATGTCTAGCATGGTG - 3' |
| C_split_TEV_rv | 5'- CCGCTACTAGTATTAACCGG - 3' |
| PIF6_2-100_fw | 5'- AAGCTTTCTAGATGGCCGGCATGTTCTTACCAACCGATTATTGTTGC - 3' |
| PIF6_2-100_rv | 5'- TTCGAAACCGGTGTCAACATGTTTATTGCTTTC - 3' |
| MinMCS_fo | 5'- CTAGACCTAGGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3' |
| MinMCS_rev | 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCCTAGGT - 3' |
| Bpu_for | 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3' |
| Bpu_rev | 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3' |
| Bpu_SDMI_for | 5'- CACTTAAGCATTTTCTACGGGTCGATCACACCATTCA - 3' |
| Bpu_SDMI_rev | 5'- TGAATGGTGTGATCGACCCGTAGAAAATGCTTAAGTG - 3' |
| Bpu_SDMII_fo | 5'- GACACGATTAAATGTGTATGCGGGCTTAGCTGGATTTTATTTG - 3' |
| Bpu_SDMII_re | 5'- CAAATAAAATCCAGCTAAGCCCGCATACACATTTAATCGTGTC - 3' |
| Bpu_SDMIII_fo | 5'- CACCCTTAAAAATAAAGCTGGCTGCGGACAGGAAG - 3' |
| Bpu_SDMIII_re | 5'- TTCCTGTCCGCAGCCAGCTTTATTTTTAAGGGTG - 3' |
| Bpu_SDMIV_fo | 5'- CGTACGCTCACTCTTACGGGTACACAGGATAAATA - 3' |
| Bpu_SDMIV_re | 5'- TATTTATCCTGTGTACCCGTAAGAGTGAGCGTACG - 3' |
| xylE_for | 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3' |
| xylE_rev | 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3' |
| xylE_c312a_for | 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3' |
| xylE_c312a_rev | 5'- GCGCACGCGCCGTCCACAACTGTTC - 3' |
| xylE_g483a_for | 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3' |
| xylE_g483a_rev | 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3' |
| xylE_a834g_for | 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3' |
| xylE_a834_rev | 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3' |
| p Act_for | 5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3' |
| pAct_rev | 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3' |
| t35S_for | 5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3' |
| t35S_rev | 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3' |
| Npt_for | 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3' |
| Npt_rev | 5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3' |
| miniMCSII_fw | 5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3' |
| miniMCSII_rv | 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3' |
| SpyTag_fw | 5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3' |
| SpyTag_rv | 5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3' |
| PIF3(2-100)_fw | 5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3' |
| PIF3(2-100)_rv | 5'- CCGCTACTAGTATTAACCGG - 3' |
| NucA_fw | 5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3' |
| NucA_rv | 5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3' |
| AlcR_fw | 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3' |
| AlcR_rv | 5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3' |
| SQ_pAutoRex8_1 | 5'- CGGGGCATCACAGCGTCG - 3' |
| SQ_pAutoRex8_2 | 5'- TATCGGTAACCGAGTCCG - 3' |
| AlcR_QCI_fw | 5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3' |
| AlcR_QCI_rv | 5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3' |
| AlcR_QCII_fw | 5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3' |
| AlcR_QCII_rv | 5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3' |
| AlcR_QCIII_fw | 5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3' |
| AlcR_QCIII_rv | 5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3' |
| AlcR_QCIV_fw | 5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3' |
| AlcR_QCIV_rv | 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3' |
| pActin_QC_fw | 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3' |
| pActin_QC_rv | 5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3' |
| NPT_QCI_fw | 5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3' |
| NPT_QCI_rv | 5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3' |
| NPT_QCII_fw | 5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3' |
| NPT_QCII_rv | 5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3' |
| IRES_polio_fw | 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3' |
| IRES_polio_rv | 5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3' |
| BPU_SDMI_r_for | 5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3' |
| BPU_SDMI_r_rev | 5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3' |
| Strep-TEV_for | 5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3' |
| Strep-TEV_rev | 5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3' |
| SQ_pluc-Actin_1 | 5'- CCGACTCAAATACAGATATGC - 3' |
| SQ_pluc-Actin_2 | 5'- CTCGGATGTAGATCTGCG - 3' |
| pActin_QC_ins_fw | 5'- ACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACC |
| pActin_QC_ins_rv | 5'- CGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGT |
| pActin_Oryza_fw | 5'- AAGCTTTCTAGAGTAGCTAGCATACTCGAGGTCATTCATATGCTTG - 3' |
| pActin_Oryza_rv | 5'- TTCGAACTGCAGCGGCCGCTACTAGTACTTCTACCTACAAAAAAGCTCCG - 3' |
| GGGGSx5_TEV_fw | 5'- CTAGATGGCCGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGGAGG |
| GGGGSx5_TEV_rv | 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCTGAGCCTCCACCTCCGGAGCCACCGCCTCCC |
| mMCSII_fw | 5'- AATTCGCGGCCGCTTCTAGAGCAATTGAAGCTTCCTGCAGGTA - 3' |
| mMCSII_rv | 5'- CTAGTACCTGCAGGAAGCTTCAATTGCTCTAGAAGCGGCCGCG - 3' |
| FluA_tripmut_fw | 5'- AAGCTTTCTAGATGGCCGGCGACGTGTACCACGACGGTG - 3' |
| FluA_tripmut_rv | 5'- TTCGAAACCGGTATTGTTGACCTTGCAGGCGG - 3' |
| pActin_QCI_fw | 5'- CATAATTTTTGAGAAAAATATATATTCAGGCGAATTGTCACAATGAACAATAATAAGATTAAAATA - 3' |
| pActin_QCI_rv | 5'- TATTTTAATCTTATTATTGTTCATTGTGACAATTCGCCTGAATATATATTTTTCTCAAAAATTATG - 3' |
| Alc R fw Seq. | 5'- CAGAGCACAATTGTCCATAC - 3' |
| Alc R rv Seq. | 5'- TCGCGGTCGATGCTCTCC - 3' |
| pActin_QCins_fw | 5'- CATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCAC |
| pActin_QCins_rv | 5'- GAGGAGCTCGTCGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGG |
| PP1_for | 5'- GCCGCTTCTAGATGGCCGGCGGATCCGCGGATTTAGATA - 3' |
| PP1_rev | 5'- GTATTAACCGGTTTTCTTTGCTTGCTTTGTGATC - 3' |
| PhyB fw Seq | 5'- GCGATTTCTCAGTTACAGGC - 3' |
| PhyB rv Seq. | 5'- CTCAAAGCACGAGAAAGAAGC - 3' |
| pAct_ins_fw | 5'- ACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTC - 3' |
| pAct_ins_rv | 5'- GGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATGG - 3' |
| PppAct_fw | 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATCGAGTCCTTGTGACTTTTGTGCAT - 3' |
| PppAct_rv | 5'- TTCGAAACTAGTATATTTAATACGGACCTGCACAACAACCACCAAT - 3' |
| PppActin_QC1_fw | 5'- TTATTGAGGATATCCATCTATCTTGATTTTGGACAATGTTTTACTGC - 3' |
| PppActin_QC1_rv | 5'- GCAGTAAAACATTGTCCAAAATCAAGATAGATGGATATCCTCAATAA - 3' |
| PppActin_QC2_fw | 5'- ACACTCTACATTCATGTCAAGAGTATAGGGACTTGGG - 3' |
| PppActin_QC2_rv | 5'- CCCAAGTCCCTATACTCTTGACATGAATGTAGAGTGT - 3' |
| PppActin_fw_corr | 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGCGAGTCCTTGTGACTTTTGTGCAT - 3' |
| SYFP2_fw | 5'- AAGCTTTCTAGATGGCCGGCGTTAGCAAGGGCGAAGAAC - 3' |
| SYFP2_rv | 5'- TTCGAAACCGGTTTTATACAGCTCATCCATACCCAG - 3' |
| eCFP_fw | 5'- AAGCTTTCTAGATGGCCGGCGTGAGCAAGGGCGAGGAG - 3' |
| eCFP_rv | 5'- TTCGAAACCGGTCTTGTACAGCTCGTCCATGCC - 3' |
| G-TEV-site-G_fw | 5'- CTAGATGGCCGGCGGAGAGAATTTGTATTTTCAGTCAGGAA - 3' |
| G-TEV-site-G_fw | 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCGCCGGCCAT - 3' |
| PppActin_seq_fw | 5'- CATGTCCCAGGGCCCTAG - 3' |
Instruments
Electrophoresis
Photometers
Nanodrop Spectrohpotometer
Moss-cultivation
References:
http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984
- http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
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iGEM Team TU-Munich
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Email: igem@wzw.tum.de
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