Team:TU-Munich/Project/Bioaccumulation

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(Difference between revisions)
(→The Fluorescein binding Anticalin FluA)
(→Variants of the fluorescein binding Anticalin FluA)
Line 26: Line 26:
|-
|-
|Proteinvariant
|Proteinvariant
-
|K<sub>D</sub> of FluA to fluorescein [nM]
+
|K<sub>D</sub> of FluA to fluorescein
|Literature reference
|Literature reference
|BioBrick
|BioBrick
|-
|-
|FluA
|FluA
-
|
+
|152 nM
|[[http://www.ncbi.nlm.nih.gov/pubmed/10051566 Beste et al., 1999]]
|[[http://www.ncbi.nlm.nih.gov/pubmed/10051566 Beste et al., 1999]]
|<partinfo>BBa_K157004</partinfo>
|<partinfo>BBa_K157004</partinfo>
|-
|-
|FluA (R95K)
|FluA (R95K)
-
|
+
|64 nM
-
|
+
|[[http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel et al., 2005]]
|not availible as BioBrick
|not availible as BioBrick
|-
|-
|FluA (R95K, A45I, S114T)
|FluA (R95K, A45I, S114T)
-
|
+
|2 nM
|[[http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel et al., 2005]]
|[[http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel et al., 2005]]
|<partinfo>BBa_K1159002</partinfo>
|<partinfo>BBa_K1159002</partinfo>

Revision as of 14:40, 4 July 2013


BioAccumulation

BioAccumulation is a possibility to remove xenobiotics from the environment by binding them to a protein that has been designed for this purpose.
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The Fluorescein binding Anticalin FluA

There are a broad range of natural as well as engineered binding proteins availible. The most commonly known binding proteins are antibodies which defend mammals against pathogens and toxins. Beside these natural binding proteins there are more and more designed binding proteins such as (1) Anticalins based on a lipocalin scaffold (Ref), (2) Affibodies derived from the z-domain of the antibody-binding protein A (Ref) and (3) DARPins that are based on an ankyrin scaffold.

Table 1:

Variants of the fluorescein binding Anticalin FluA

Proteinvariant KD of FluA to fluorescein Literature reference BioBrick
FluA 152 nM http://www.ncbi.nlm.nih.gov/pubmed/10051566 Beste et al., 1999 <partinfo>BBa_K157004</partinfo>
FluA (R95K) 64 nM http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel et al., 2005 not availible as BioBrick
FluA (R95K, A45I, S114T) 2 nM http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel et al., 2005 <partinfo>BBa_K1159002</partinfo>
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Glutathione S-transferase

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Protein Phosphotase 1 - A molecular mop for Microcystin

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figure




References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
  2. http://www.ncbi.nlm.nih.gov/pubmed/10051566 Beste et al., 1999 Beste G, Schmidt FS, Stibora T, Skerra A. (1999) Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold. PNAS, 96(5):1898-903.
  3. http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel et al., 2005 Vopel S, Mühlbach H, Skerra A. (2005) Rational engineering of a fluorescein-binding anticalin for improved ligand affinity. Biol. Chem., 386(11):1097-104.



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