Team:TU-Munich/Project/Bioaccumulation

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BioAccumulation

BioAccumulation is a possibility to remove xenobiotics from the environment by binding them to a protein that has been designed for this purpose.
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The Fluorescein binding Anticalin FluA

There are a broad range of natural as well as engineered binding proteins availible. The most commonly known binding proteins are antibodies which defend mammals against pathogens and toxins. Beside these natural binding proteins there are more and more designed binding proteins such as (1) Anticalins based on a lipocalin scaffold (Ref), (2) Affibodies derived from the z-domain of the antibody-binding protein A (Ref) and (3) DARPins that are based on an ankyrin scaffold.

Table 1:

Variants of the fluorescein binding Anticalin FluA

Variant Affinity Literature reference BioBrick
FluA http://www.ncbi.nlm.nih.gov/pubmed/10051566 Beste et al., 1999 <partinfo>BBa_K157004</partinfo>
FluA (R95K) not availible as BioBrick
FluA (R95K, A45I, S114T) <partinfo>BBa_K1159002</partinfo>
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Glutathione S-transferase

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Protein Phosphotase 1 - A molecular mop for Microcystin

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References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
  2. http://www.ncbi.nlm.nih.gov/pubmed/10051566 Beste et al., 1999 Beste G, Schmidt FS, Stibora T, Skerra A. (1999) Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold. PNAS, 96(5):1898-903.



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