Team:TU-Munich/Project/Killswitch
From 2013.igem.org
Red light triggered self-destruction – a new mechanism preventing uncontrolled spread of transgenic plants
Background – Why generating a plant which kill itself under certain conditions?
Our solution – a red light-triggered autokill system
PhyB - PIF3/PIF6 interaction under red light exposition
Phytochrome B (abbrev.: PhyB) is a plant adölfkjadölkfjafölkjasfölkasjdf
Reconstitution of Split-TEV-Protease
Liberation of membrane-anchored nuclease and nuclear translocation
Disruption of the genetic material and programmed cell-death
Design of our construct
Results
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References:
http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984
- http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
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