Team:TU-Munich/Project/Killswitch

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Red light triggered self-destruction – a new mechanism preventing uncontrolled spread of transgenic plants

Background – Why generating a plant which kill itself under certain conditions?

Our solution – a red light-triggered autokill system

PhyB - PIF3/PIF6 interaction under red light exposition

Phytochrome B (abbrev.: PhyB) is a plant adölfkjadölkfjafölkjasfölkasjdf

Reconstitution of Split-TEV-Protease

Liberation of membrane-anchored nuclease and nuclear translocation

Disruption of the genetic material and programmed cell-death

Design of our construct

Results

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References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.