Team:TU-Munich/Project/Killswitch

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Red light triggered self-destruction – a new mechanism preventing uncontrolled spread of transgenic plants

Background – Why generating a plant which kill itself under certain conditions?

Our solution – a red light-triggered autokill system

PhyB - PIF3/PIF6 interaction under red light exposition

Phytochrome B (abbrev.: PhyB) is a plant adölfkjadölkfjafölkjasfölkasjdf

Reconstitution of Split-TEV-Protease

Liberation of membrane-anchored nuclease and nuclear translocation

Disruption of the genetic material and programmed cell-death

Design of our construct

Results

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References:

[Edens et al., 1984]

  1. [Edens et al., 1984] Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.