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Team:TU-Munich/Project/Overview
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== Project Overview == | == Project Overview == | ||
| - | In the 2013 competition the TU Munich iGEM team has developed a transgenic moss filter which is capable to reduct | + | In the 2013 competition the TU Munich iGEM team has developed a transgenic moss filter which is capable to reduct the contamination of aquatic ecosystems with xenobiotics. The topic of remediation using transgenic organisms is present in every year of iGEM and we want to take this idea to the next level. For this reason we decided to use a photoautotrophic chassis which we enabled for the iGEM competition. The following section gives an overview over the theoretical background of our project. |
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===''Physcomitrella''=== | ===''Physcomitrella''=== | ||
[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 2:]] | [[File:TUM13 Icon.jpg|thumb|right|200px| Figure 2:]] | ||
| - | + | Next, we introduce ''Physcomitrella patens'' as a nearly new chassis to iGEM. We describe advantages, possiblilities and describe our expression strategy ([https://2013.igem.org/Team:TU-Munich/Project/Physcomitrella Read more]). | |
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===Localisation=== | ===Localisation=== | ||
[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 3:]] | [[File:TUM13 Icon.jpg|thumb|right|200px| Figure 3:]] | ||
| - | As the principle of action for the effectors responsible for the Phytoremediation are very different it is necessary to have a expression system which is able to accomplish cytosolic, secreted | + | As the principle of action for the effectors, responsible for the Phytoremediation, are very different it is necessary to have a expression system which is able to accomplish cytosolic, secreted and receptor bound localization of proteins ([https://2013.igem.org/Team:TU-Munich/Project/Localisation Read more]). |
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===BioDegradation=== | ===BioDegradation=== | ||
[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 4:]] | [[File:TUM13 Icon.jpg|thumb|right|200px| Figure 4:]] | ||
| - | BioDegradation describes the application of enzymatic catalysis for the degradation of problematic xenobiotics using | + | BioDegradation describes the application of enzymatic catalysis for the degradation of problematic xenobiotics using the enzymes erythromycin esterase, catechol dioxigenase and laccase ([https://2013.igem.org/Team:TU-Munich/Project/Biodegradation Read more]). |
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===Safety=== | ===Safety=== | ||
[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 5:]] | [[File:TUM13 Icon.jpg|thumb|right|200px| Figure 5:]] | ||
| - | Our safety page describes our | + | Our safety page describes our team discussions concerning safty issues, especially a safety evaluation of our BioBricks and ''Physcomitralla'' including all constructed GM-Moss plants([https://2013.igem.org/Team:TU-Munich/Project/Safety Read more]). |
</div> | </div> | ||
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===Kill-switch=== | ===Kill-switch=== | ||
[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 6:]] | [[File:TUM13 Icon.jpg|thumb|right|200px| Figure 6:]] | ||
| - | In order to restrict the growth of transgenic moss to a controlled environment we developed a kill-switch which | + | In order to restrict the growth of transgenic moss to a controlled environment, we developed a kill-switch which is triggered by sunlight. The GM-Moss can only be grown where red-light is filtered out of the electromagnetic spectrum ([https://2013.igem.org/Team:TU-Munich/Project/Killswitch Read more]). |
</div> | </div> | ||
Revision as of 09:04, 4 October 2013
Project Overview
In the 2013 competition the TU Munich iGEM team has developed a transgenic moss filter which is capable to reduct the contamination of aquatic ecosystems with xenobiotics. The topic of remediation using transgenic organisms is present in every year of iGEM and we want to take this idea to the next level. For this reason we decided to use a photoautotrophic chassis which we enabled for the iGEM competition. The following section gives an overview over the theoretical background of our project.
Phytoremediation
Phytoremediation describes the treatment of environmental problems through the use of plants. Here we identified problematic substances and described basic principles (Read more).
Physcomitrella
Next, we introduce Physcomitrella patens as a nearly new chassis to iGEM. We describe advantages, possiblilities and describe our expression strategy (Read more).
Localisation
As the principle of action for the effectors, responsible for the Phytoremediation, are very different it is necessary to have a expression system which is able to accomplish cytosolic, secreted and receptor bound localization of proteins (Read more).
BioDegradation
BioDegradation describes the application of enzymatic catalysis for the degradation of problematic xenobiotics using the enzymes erythromycin esterase, catechol dioxigenase and laccase (Read more).
Safety
Our safety page describes our team discussions concerning safty issues, especially a safety evaluation of our BioBricks and Physcomitralla including all constructed GM-Moss plants(Read more).
Kill-switch
In order to restrict the growth of transgenic moss to a controlled environment, we developed a kill-switch which is triggered by sunlight. The GM-Moss can only be grown where red-light is filtered out of the electromagnetic spectrum (Read more).
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