Team:TU-Munich/Results/GM-Moss

From 2013.igem.org

(Difference between revisions)
(→Transformation of Physcomitrella patens)
(→Transformation of Physcomitrella patens)
Line 12: Line 12:
[[File:TUM13_Workflow_Transformation_detailled.png|thumb|center|900px|'''Figure 2''': Work flow for the generation of transgenic moss.]]
[[File:TUM13_Workflow_Transformation_detailled.png|thumb|center|900px|'''Figure 2''': Work flow for the generation of transgenic moss.]]
-
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
+
<br><br><br><br><br><br><br><br><br><br><br>
</div>
</div>

Revision as of 21:35, 25 September 2013



Transformation of Physcomitrella patens

Figure 1: Work flow for the generation of transgenic moss.
Figure 2: Work flow for the generation of transgenic moss.












1 Generation of expression constructs

Table 1:

Transgenic Physcomitrella patnes plants

Number Construct name (abbreviation) BioBrick Figure Size [bp] Lane
PF-1 GFP (GFP-cyt) <partinfo>BBa_E0040</partinfo> x
PF-2 Igk-GFP (GFP-sec) <partinfo>BBa_E0040</partinfo> x
PF-3 NanoLuciferase (nLuc-cyt) <partinfo>BBa_E0040</partinfo> x
PF-4 Igk-NanoLuciferase (nLuc-sec) <partinfo>BBa_E0040</partinfo> x
PF-5 SERK-NanoLuciferase (SERK-nLuc) x
PF-6 NanoLuciferase-Receptor (nLuc-rec) <partinfo>BBa_E0040</partinfo> x
PF-7 Erythromycinesterase (EreB-cyt) <partinfo>BBa_E0040</partinfo> x
PF-8 Erythromycinesterase-Receptor (EreB-rec) <partinfo>BBa_E0040</partinfo> #
PF-9 Ig Kappa Erythromycinesterase (EreB-sec) <partinfo>BBa_E0040</partinfo> #
PF-10 Laccase-Receptor (Lac-rec) #
PF-11 Ig Kappa Laccase (Lac-sec) #
PF-12 Catecholdioxygenase (XylE-cyt) <partinfo>BBa_E0040</partinfo> x
PF-13 DDT-dehydrochlorinase (GST-cyt) <partinfo>BBa_E0040</partinfo> #
PF-14 PP1-Receptor (PP1-rec) <partinfo>BBa_E0040</partinfo> x
PF-15 FluA-Receptor (FluA-rec) <partinfo>BBa_E0040</partinfo> x
PF-16 Stressinducible_Promoter-RFP (Stress) <partinfo>BBa_E0040</partinfo> #
PF-17 SpyCatcher-Receptor:Spytag-nLuc (Catcher:Tag-nLuc) <partinfo>BBa_E0040</partinfo> #
PF-18 SpyCatcher-Receptor:nLuc-Spytag (Catcher:nLuc-Tag <partinfo>BBa_E0040</partinfo> #
PF-19 SpyTag-Receptor:SpyCatcher-nLuc (Tag:Catcher-nLuc) <partinfo>BBa_E0040</partinfo> #
PF-20 SpyTag-Receptor:nLuc-SpyCatcher (Tag:nLuc-Catcher) <partinfo>BBa_E0040</partinfo> #
PF-21 Alcohol acetyltransferase I (Banana) <partinfo>BBa_J45014</partinfo> #
PF-22 Kill-switch with PIF3 (PIF-3) <partinfo>BBa_E0040</partinfo> x
PF-23 Kill-switch with PIF6 (PIF-6) <partinfo>BBa_E0040</partinfo> x
PF-24 Kill-switch with PIF6 (FRET) <partinfo>BBa_E0040</partinfo> #
PF-25 Kill-switch with PIF3 (FRET) <partinfo>BBa_E0040</partinfo> #


2 Preparation of linear DNA

3 Transformation of Physcomitrella patens


4 Regeneration and Selektion of transgenic plants

5 Characterization of transgenic plants


References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.



Retrieved from "http://2013.igem.org/Team:TU-Munich/Results/GM-Moss"