Team:TU-Munich/Results/GM-Moss

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Transformation of Physcomitrella patens

Figure 1: Work flow for the generation of transgenic moss.






















1 Generation of expression constructs

Table 1:

Transgenic Physcomitrella patnes plants

Number Construct name (abbreviation) BioBrick Figure Size [bp] Lane
PF-1 GFP (GFP-cyt) <partinfo>BBa_E0040</partinfo> x
PF-2 Igk-GFP (GFP-sec) <partinfo>BBa_E0040</partinfo> x
PF-3 NanoLuciferase (nLuc-cyt) <partinfo>BBa_E0040</partinfo> x
PF-4 Igk-NanoLuciferase (nLuc-sec) <partinfo>BBa_E0040</partinfo> x
PF-5 SERK-NanoLuciferase (SERK-nLuc) x
PF-6 NanoLuciferase-Receptor (nLuc-rec) <partinfo>BBa_E0040</partinfo> x
PF-7 Erythromycinesterase (EreB-cyt) <partinfo>BBa_E0040</partinfo> x
PF-8 Erythromycinesterase-Receptor (EreB-rec) <partinfo>BBa_E0040</partinfo> #
PF-9 Ig Kappa Erythromycinesterase (EreB-sec) <partinfo>BBa_E0040</partinfo> #
PF-10 Laccase-Receptor (Lac-rec) #
PF-11 Ig Kappa Laccase (Lac-sec) #
PF-12 Catecholdioxygenase (XylE-cyt) <partinfo>BBa_E0040</partinfo> x
PF-13 DDT-dehydrochlorinase (GST-cyt) <partinfo>BBa_E0040</partinfo> #
PF-14 PP1-Receptor (PP1-rec) <partinfo>BBa_E0040</partinfo> x
PF-15 FluA-Receptor (FluA-rec) <partinfo>BBa_E0040</partinfo> x
PF-16 Stressinducible_Promoter-RFP (Stress) <partinfo>BBa_E0040</partinfo> #
PF-17 SpyCatcher-Receptor:Spytag-nLuc (Catcher:Tag-nLuc) <partinfo>BBa_E0040</partinfo> #
PF-18 SpyCatcher-Receptor:nLuc-Spytag (Catcher:nLuc-Tag <partinfo>BBa_E0040</partinfo> #
PF-19 SpyTag-Receptor:SpyCatcher-nLuc (Tag:Catcher-nLuc) <partinfo>BBa_E0040</partinfo> #
PF-20 SpyTag-Receptor:nLuc-SpyCatcher (Tag:nLuc-Catcher) <partinfo>BBa_E0040</partinfo> #
PF-21 Alcohol acetyltransferase I (Banana) <partinfo>BBa_J45014</partinfo> #
PF-22 Kill-switch with PIF3 (PIF-3) <partinfo>BBa_E0040</partinfo> x
PF-23 Kill-switch with PIF6 (PIF-6) <partinfo>BBa_E0040</partinfo> x
PF-24 Kill-switch with PIF6 (FRET) <partinfo>BBa_E0040</partinfo> #
PF-25 Kill-switch with PIF3 (FRET) <partinfo>BBa_E0040</partinfo> #


2 Preparation of linear DNA

3 Transformation of Physcomitrella patens


References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.



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