Team:TU-Munich/Results/Software/FeatureList

From 2013.igem.org

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===Tag and binding sites===
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===Tags and binding sites===
*Arg5-tag: RRRRR
*Arg5-tag: RRRRR
*''Strep''-tag I: AWRHPQFGG
*''Strep''-tag I: AWRHPQFGG
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*SBP-tag: MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP
*SBP-tag: MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP
*Avitag: GLNDIFEAQKIEWHE
*Avitag: GLNDIFEAQKIEWHE
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===Cleavage sites===
===Cleavage sites===
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*PreScission cleavage site: LEVLFQGP
*PreScission cleavage site: LEVLFQGP
*TEV cleavage site: ENLYFQG
*TEV cleavage site: ENLYFQG
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*TEV cleavage site: ENLYFQS
*TVMV cleavage site: ETVRFQGS
*TVMV cleavage site: ETVRFQGS
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===Localization Signals===
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*SV40 nuclear localization sequence: PKKKRKV
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===Other features===
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*[http://parts.igem.org/Assembly_standard_25 RFC 25] scar: In-frame ACCGGC (in nucleotide sequence), translating to TG
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Latest revision as of 09:32, 24 September 2013


Tags and binding sites

  • Arg5-tag: RRRRR
  • Strep-tag I: AWRHPQFGG
  • Strep-tag II: WSHPQFEK
  • Flag-tag: DYKDDDDK
  • 3xFlag-tag: DYKDHDGDYKDHDIDYKDDDDK
  • HA-tag: YPYDVPDYA
  • His5-tag: HHHHH
  • His6-tag: HHHHHH
  • c-Myc-tag: EQKLISEEDL
  • S-tag: KETAAAKFERQHMDS
  • HAT-tag: KDHLIHNVHKEFHAHAHNK
  • Calmodulin-binding-peptide: KRRWKKNFIAVSAANRFKKISSSGAL
  • Isopep-tag: TDKDMTITFTNKKDAE
  • Spy-tag: AHIVMVDAYKPTK
  • Chitin-binding domain: TNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ
  • SBP-tag: MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP
  • Avitag: GLNDIFEAQKIEWHE

Cleavage sites

  • Enterokinase cleavage site: DDDDK
  • Factor Xa cleavage site: ID/EGR
  • Thrombin cleavage site: LVPRGS
  • PreScission cleavage site: LEVLFQGP
  • TEV cleavage site: ENLYFQG
  • TEV cleavage site: ENLYFQS
  • TVMV cleavage site: ETVRFQGS

Localization Signals

  • SV40 nuclear localization sequence: PKKKRKV

Other features

  • RFC 25 scar: In-frame ACCGGC (in nucleotide sequence), translating to TG


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