Team:USTC CHINA/Parts

From 2013.igem.org

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         <h1>PHT43</h1>
         <h1>PHT43</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2>
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         <p align="center">PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis... </p>
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         <p align="center">PHT43 is a <i>E.coli-B.subtilis</i> shuttle vector allowing high-level expression of secreted proteins in <i>B.subtilis</i>... </p>
</div>
</div>
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         <h1>LTB</h1>
         <h1>LTB</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2>  
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         <p align="center">This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli...</p>
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         <p align="center">This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of <i>E.coli</i>...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
         <h1>Pctc+RBS+<br>amilCP</h1>
         <h1>Pctc+RBS+<br>amilCP</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2>  
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         <p align="center">Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. activated by endogenous sigma factor B ...</p>
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         <p align="center">Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in <i>B.subtilis</i> activated by endogenous sigma factor B ...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
         <h1>SdpA,SdpB,<br>SpbC</h1>
         <h1>SdpA,SdpB,<br>SpbC</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2>  
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         <p align="center">Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway...</p>
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         <p align="center">Killing factor of <i>B.subtilis</i> ,SpbC Induces the lysis of other <i>B.subtilis</i> cells that have not entered the sporulation pathway...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
         <h1>Pgrac+RBS+SDP</h1>
         <h1>Pgrac+RBS+SDP</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2>  
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         <p align="center">Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of Bacillus subtilis...</p>
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         <p align="center">Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of <i>B.subtilis</i>...</p>
</div>
</div>
<div class="clear"></div>
<div class="clear"></div>
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         <h1>PsdpRI+RBS<br>+SDP</h1>
         <h1>PsdpRI+RBS<br>+SDP</h1>
       <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2>  
       <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2>  
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         <p align="center">We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose...</p>
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         <p align="center">We construct this gene circuit as our kill switch to kill the engineered <i>B.subtilis</i> for the safety purpose...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
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         <h1>promoter sdpR/I</h1>
         <h1>promoter sdpR/I</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2>  
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         <p align="center">This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis...</p>
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         <p align="center">This part is a sigma factor A-dependent promotor of the gene sdpR derived from the <i>B.subtilis</i>...</p>
</div>
</div>
<div class="part-col-2">
<div class="part-col-2">
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         <h1>SamyQ</h1>
         <h1>SamyQ</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074014">BBa_K1074014</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074014">BBa_K1074014</a></h2>  
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         <p align="center">SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in Bacillus subtilis WB800N...</p>
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         <p align="center">SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in <i>B.subtilis</i> WB800N...</p>
</div>
</div>
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         <h1>RBS for Pgrac</h1>
         <h1>RBS for Pgrac</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2>  
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         <p align="center">An efficient RBS for promoter Pgrac(BBa_K1074012) in Bacillus subtilis.../p>
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         <p align="center">An efficient RBS for promoter Pgrac(BBa_K1074012) in <i>B.subtilis</i>.../p>
</div>
</div>
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         <h1>RBS for Pctc</h1>
         <h1>RBS for Pctc</h1>
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2>  
         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2>  
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         <p align="center">This is an efficient RBS for promoter Pctc(BBa_K143010) in Bacillus subtilis...</p>
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         <p align="center">This is an efficient RBS for promoter Pctc(BBa_K143010) in <i>B.subtilis</i>...</p>
</div>
</div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></div>
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<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">Furthur Work</a></div>
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<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/AdvancingWork">Advancing Work</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/FutureWork">Future Work</a></div>
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
</div></div></div>         
</div></div></div>         

Latest revision as of 12:33, 28 October 2013

TD1, Transdermal peptide

BBa_K1074000

TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...

Pgrac+RBS+SamyQ+TD1
+GFP

BBa_K1074006

This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...

PHT43

BBa_K1074001

PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis...

HBsAg

BBa_K1074002

HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...

Ag85b

BBa_K1074003

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin...

Protective Antigen Domain 4

BBa_K1074004

Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax...

LTB

BBa_K1074005

This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli...

Pctc+RBS+
amilCP

BBa_K1074007

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B.subtilis activated by endogenous sigma factor B ...

SdpA,SdpB,
SpbC

BBa_K1074009

Killing factor of B.subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway...

Pgrac+RBS+SDP

BBa_K1074010

Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of B.subtilis...

PsdpRI+RBS
+SDP

BBa_K1074011

We construct this gene circuit as our kill switch to kill the engineered B.subtilis for the safety purpose...

Pgrac promoter

BBa_K1074012

Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG...

promoter sdpR/I

BBa_K1074013

This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis...

SamyQ

BBa_K1074014

SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in B.subtilis WB800N...

RBS for Pgrac

BBa_K1074015

An efficient RBS for promoter Pgrac(BBa_K1074012) in B.subtilis.../p>

RBS for Pctc

BBa_K1074016

This is an efficient RBS for promoter Pctc(BBa_K143010) in B.subtilis...