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Team:USTC CHINA/Parts
From 2013.igem.org
TD1, Transdermal peptide
BBa_K1074000
TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin. Studies suggested that the peptide creates a transient opening in the skin barrier to enable macromolecular material to reach systemic circulation.
Pgrac+RBS+SamyQ+TD1
+GFP
BBa_K1074006
This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method.
PHT43
BBa_K1074001
PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis.
HBsAg
BBa_K1074002
HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection.
Ag85b
BBa_K1074003
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs).
Protective Antigen Domain 4
BBa_K1074004
Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax.
LTB
BBa_K1074005
This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli.
Pctc+RBS+amilCP
BBa_K1074007
Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. activated by endogenous sigma factor B under mild stress( nutrient stress response or physical stress response).
SdpA,SdpB,SpbC
BBa_K1074009
Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation.
Pgrac+RBS+SDP
BBa_K1074010
Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of Bacillus subtilis.
PsdpRI+RBS+SDP
BBa_K1074011
We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose.
Pgrac promoter
BBa_K1074012
Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG.
promoter sdpR/I
BBa_K1074013
This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis ,and it contains an operon for the signaling pathway of sdpc,a bacterial toxin of B.subtilis.
SamyQ
BBa_K1074014
SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in Bacillus subtilis WB800N. Fuse it with the recombinant proteins to obtain the secretion of recombinant proteins in Bacillus subtilis.
RBS for Pgrac
BBa_K1074015
An efficient RBS for promoter Pgrac(BBa_K1074012) in Bacillus subtilis. We used it in all of our gene circuits containing promoter Pgrac.
RBS for Pctc
BBa_K1074016
This is an efficient RBS for promoter Pctc(BBa_K143010) in Bacillus subtilis. It's also an universal RBS for promoters in Bacillus subtilis. We also used it with the promoter PsdpRI(BBa_K1074013) in our project.
