Team:USTC CHINA/Project/AdvancingWork
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(Difference between revisions)
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<div class="atfigure" align="center" style="width:580px;font-size:14px;">Fig4. fluorescent microscope shows the expression of GFP in WB800N</div> | <div class="atfigure" align="center" style="width:580px;font-size:14px;">Fig4. fluorescent microscope shows the expression of GFP in WB800N</div> | ||
<div><h3>2.Expression of TD1-antigen/adjuvant</h3></div> | <div><h3>2.Expression of TD1-antigen/adjuvant</h3></div> | ||
- | + | <p align="justify">In order to realize the secretory expression in B.subtilis, we inserted signal peptide between promoter and the TD1-Antigen/adjuvant sequence to secrete our recombinant protein. GFP was chosen to check the reliability of this circuit. After large amounts of experiments, GFP has finally been found via fluorescence microscope.</p> | |
<img src="https://static.igem.org/mediawiki/2013/f/f0/Pctc-sp-td1-antigenadjuvent.png"width="580" height="120" /> | <img src="https://static.igem.org/mediawiki/2013/f/f0/Pctc-sp-td1-antigenadjuvent.png"width="580" height="120" /> | ||
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<div><p align="justify">We have figured it out that the best concentration of neomycin for selection use is 128mg/ml.</p></div> | <div><p align="justify">We have figured it out that the best concentration of neomycin for selection use is 128mg/ml.</p></div> | ||
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- | + | <h2>THREE: Mice Experiment is in Progress</h2> | |
<h3>Specific Pathogen Free (SPF) Laboratory</h3> | <h3>Specific Pathogen Free (SPF) Laboratory</h3> | ||
<div><p align="justify">Specific Pathogen Free is a term used for laboratory animals that are guaranteed free of particular pathogens. Use of SPF animals ensures that specified diseases do not interfere with an experiment. For example, absence of respiratory pathogens such as influenza is desirable when investigating a drug's effect on lung function.</p></div> | <div><p align="justify">Specific Pathogen Free is a term used for laboratory animals that are guaranteed free of particular pathogens. Use of SPF animals ensures that specified diseases do not interfere with an experiment. For example, absence of respiratory pathogens such as influenza is desirable when investigating a drug's effect on lung function.</p></div> |
Revision as of 11:13, 28 October 2013